环磷腺苷依赖性蛋白激酶催化亚基α在慢性髓系白血病中的表达及其临床意义
Complex analysis of cyclin D1 expression in mantle cell lymphoma: two cyclin D1-negative cases detected.
机构信息
Department of Pathology, University Hospital Brno Jihlavska 20, 625 00 Brno, Czech Republic.
出版信息
J Clin Pathol. 2009 Oct;62(10):948-50. doi: 10.1136/jcp.2008.063701.
BACKGROUND AND AIM
The cytogenetic and diagnostic hallmark of mantle cell lymphoma (MCL) is translocation t(11;14)(q13;q32), resulting in overexpression of cyclin D1. Cyclin D1 expression was analysed in 32 cases of MCL.
METHODS
The t(11;14) translocation was detected by fluorescence in situ hybridisation, level of cyclin D1 mRNA by competitive RT-PCR, and level of cyclin D1 and D2 proteins by immunohistochemistry and/or immunoblotting.
RESULTS
In 30 cases, the presence of translocation t(11;14), a high level of cyclin D1 mRNA, and a high level of the cyclin D1 protein were confirmed. Two cyclin D1-negative cases overexpressing cyclin D2 were detected by immunoblotting.
CONCLUSIONS
There are rare cyclin D1-negative cases of MCL overexpressing cyclin D2. Anti-cyclin D1 antibodies with low specificity can bind both cyclin D1 and cyclin D2, thus providing false cyclin D1-positive signals in immunohistochemical analysis.
背景与目的
套细胞淋巴瘤（MCL）的细胞遗传学和诊断标志是易位 t(11;14)(q13;q32)，导致 cyclin D1 的过表达。本研究分析了 32 例 MCL 中 cyclin D1 的表达。
方法
通过荧光原位杂交检测 t(11;14)易位，竞争性 RT-PCR 检测 cyclin D1 mRNA 的水平，免疫组化和/或免疫印迹检测 cyclin D1 和 D2 蛋白的水平。
结果
在 30 例病例中，证实存在易位 t(11;14)、cyclin D1 mRNA 水平高和 cyclin D1 蛋白水平高。通过免疫印迹检测到 2 例 cyclin D1 阴性但 cyclin D2 过表达的病例。
结论
MCL 中存在罕见的 cyclin D1 阴性但 cyclin D2 过表达的病例。特异性低的抗 cyclin D1 抗体可以与 cyclin D1 和 cyclin D2 结合，从而在免疫组化分析中提供假 cyclin D1 阳性信号。
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