Thivichon-Prince B, Couble M L, Giamarchi A, Delmas P, Franco B, Romio L, Struys T, Lambrichts I, Ressnikoff D, Magloire H, Bleicher F
University of Lyon, Villeurbanne, F-69100, France.
J Dent Res. 2009 Oct;88(10):910-5. doi: 10.1177/0022034509345822.
A primary cilium, a sensory organelle present in almost every vertebrate cell, is regularly described in odontoblasts, projecting from the surfaces of the cells. Based on the hypothesis that the primary cilium is crucial both for dentin formation and possibly in tooth pain transmission, we have investigated the expression and localization of the main cilium components and involvement of the OFD1 gene in tooth morphogenesis. Odontoblasts in vitro express tubulin, inversin, rootletin, OFD1, BBS4, BBS6, ALMS1, KIF3A, PC1, and PC2. In vivo, cilia are aligned parallel to the dentin walls, with the top part oriented toward the pulp core. Close relationships between cilium and nerve fibers are evidenced. Calcium channels are concentrated in the vicinity of the basal body. Analysis of these data suggests a putative role of cilia in sensing the microenvironment, probably related to dentin secretion. This hypothesis is enhanced by the huge defects observed on molars from Ofd1 knockout mice, showing undifferentiated dentin-forming cells.
初级纤毛是几乎存在于每一个脊椎动物细胞中的一种感觉细胞器，在成牙本质细胞中经常被描述为从细胞表面伸出。基于初级纤毛对牙本质形成以及可能对牙痛传递都至关重要的假设，我们研究了主要纤毛成分的表达和定位以及OFD1基因在牙齿形态发生中的作用。体外培养的成牙本质细胞表达微管蛋白、反转蛋白、根蛋白、OFD1、BBS4、BBS6、ALMS1、KIF3A、PC1和PC2。在体内，纤毛与牙本质壁平行排列，顶部朝向牙髓腔。纤毛与神经纤维之间存在密切关系得到了证实。钙通道集中在基体附近。对这些数据的分析表明纤毛在感知微环境中可能发挥作用，这可能与牙本质分泌有关。Ofd1基因敲除小鼠磨牙上观察到的巨大缺陷进一步支持了这一假设，这些缺陷显示出未分化的牙本质形成细胞。
