DeGasperi R, Thomas L J, Sugiyama E, Chang H M, Beck P J, Orlean P, Albright C, Waneck G, Sambrook J F, Warren C D
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Massachusetts General Hospital, Boston 02114.
Science. 1990 Nov 16;250(4983):988-91. doi: 10.1126/science.1978413.
Glycosylphosphatidylinositol (GPI) serves as a membrane anchor for a large number of eukaryotic proteins. A genetic approach was used to investigate the biosynthesis of GPI anchor precursors in mammalian cells. T cell hybridoma mutants that cannot synthesize dolichol-phosphate-mannose (Dol-P-Man) also do not express on their surface GPI-anchored proteins such as Thy-1 and Ly-6A. These mutants cannot form mannose-containing GPI precursors. Transfection with the yeast Dol-P-Man synthase gene rescues the synthesis of both Dol-P-Man and mannose-containing GPI precursors, as well as the surface expression of Thy-1 and Ly-6A, suggesting that Dol-P-Man is the donor of at least one mannose residue in the GPI core.
糖基磷脂酰肌醇（GPI）作为大量真核生物蛋白质的膜锚定物。采用遗传学方法研究哺乳动物细胞中GPI锚定前体的生物合成。不能合成磷酸多萜醇甘露糖（Dol-P-Man）的T细胞杂交瘤突变体在其表面也不表达GPI锚定蛋白，如Thy-1和Ly-6A。这些突变体不能形成含甘露糖的GPI前体。用酵母Dol-P-Man合酶基因转染可挽救Dol-P-Man和含甘露糖的GPI前体的合成，以及Thy-1和Ly-6A的表面表达，这表明Dol-P-Man是GPI核心中至少一个甘露糖残基的供体。
