Rocha Anna Silvia Setti da, Ramos-Perez Flávia Maria de Moraes, Bóscolo Frab Norberto, Manzi Flavio Ricardo, Cchicarelo Mariliani, Almeida Solange Maria
Department of Physics, Federal Technological University of Paraná, Curitiba, PR, Brazil.
Braz Dent J. 2009;20(3):186-90. doi: 10.1590/s0103-64402009000300002.
This study evaluated the radioprotective effect of sodium selenite on the bone repair process in tibiae of female rats. For such purpose, 100 female Wistar rats (Rattus norvegicus, albinus) were randomly assigned to 4 groups (n=25), according to the treatment received: administration of distilled water (control); administration of sodium selenite; gamma radiation; and administration of sodium selenite plus gamma radiation. A bone defect was prepared on both tibiae of all animals. Three days after surgery, the gamma radiation and selenium/gamma radiation groups received 8 Gy gamma rays on the lower limbs. Five animals per group were sacrificed 7, 14, 21, 28 days after surgery for evaluation of the repair process by bone volumetric density analysis. The 5 animals remaining in each group were sacrificed 45 days postoperatively for examination of the mature bone by scanning electron microscopy. Based on all analyzed parameters, the results of the present study suggest that sodium selenite exerted a radioprotective effect in the bone repair of tibia of irradiated rats.
本研究评估了亚硒酸钠对雌性大鼠胫骨骨修复过程的辐射防护作用。为此，将100只雌性Wistar大鼠（褐家鼠，白化种）根据接受的治疗随机分为4组（n = 25）：给予蒸馏水（对照组）；给予亚硒酸钠；γ射线辐射；给予亚硒酸钠加γ射线辐射。对所有动物的双侧胫骨制备骨缺损。术后3天，γ射线辐射组和硒/γ射线辐射组下肢接受8 Gy的γ射线照射。每组5只动物在术后7、14、21、28天处死，通过骨体积密度分析评估修复过程。每组剩余的5只动物在术后45天处死，通过扫描电子显微镜检查成熟骨。基于所有分析参数，本研究结果表明亚硒酸钠对受辐照大鼠胫骨的骨修复具有辐射防护作用。
