Plant Mycotoxin Research Unit, Agricultural Research Service, U. S. Dept. of Agriculture, 800 Buchanan St., Albany, CA 94710, USA.
Appl Microbiol Biotechnol. 2010 Feb;85(6):1947-52. doi: 10.1007/s00253-009-2229-8. Epub 2009 Sep 26.
To determine the effect of monensin, a carboxylic polyether ionophore antibiotic, on the bacterial population structure of dairy cattle colonic contents, we fed six lactating Holstein cows a diet containing monensin (600 mg day(-1)) or an identical diet without monensin. Fresh waste samples were taken directly from the animals once a month for 3 months and assayed for their bacterial population structure via 16S rRNA gene sequence analysis. In total 6,912 16S rRNA genes were examined, comprising 345 and 315 operational taxonomic units (OTUs) from the monensin fed and control animals, respectively. Coverage estimates of the OTUs identified were 87.6% for the monensin fed and 88.3% for the control colonic content derived library. Despite this high level of coverage, no significant difference was found between the libraries down to the genus level. Thus we concluded that although monensin is believed to increase milk production in dairy cattle by altering the bacterial population structure within the bovine gastrointestinal tract, we were unable to identify any significant difference in the bacterial population structure of the colonic contents of monensin fed vs. the control dairy cattle, down to the genus level.
为了确定莫能菌素（一种羧酸型聚醚离子载体抗生素）对奶牛结肠内容物中细菌种群结构的影响，我们给六头泌乳荷斯坦奶牛喂食含有莫能菌素（600mg/天）或不含莫能菌素的相同饮食。新鲜的废物样本每月直接从动物身上采集一次，通过 16S rRNA 基因序列分析来检测其细菌种群结构。共检查了 6912 个 16S rRNA 基因，分别来自喂食莫能菌素和对照动物的 345 和 315 个操作分类单位（OTU）。莫能菌素喂养和对照结肠内容物衍生文库中鉴定的 OTU 的覆盖率估计分别为 87.6%和 88.3%。尽管覆盖率很高，但在属水平以下，两个文库之间没有发现显著差异。因此，我们得出结论，尽管莫能菌素被认为通过改变牛胃肠道内的细菌种群结构来提高奶牛的产奶量，但我们无法确定在属水平以下，喂食莫能菌素的奶牛和对照奶牛的结肠内容物的细菌种群结构有任何显著差异。
