Laboratory of Applied Entomology, Institute of Pesticides and Environmental Protection, Banatska 31B, P.O. Box 163, 11080 Belgrade-Zemun, Serbia.
Exp Appl Acarol. 2010 Mar;50(3):255-67. doi: 10.1007/s10493-009-9316-5. Epub 2009 Sep 26.
Laboratory bioassays were conducted to evaluate the effects of spiromesifen on gross fecundity, gross fertility, net fertility and population growth of two-spotted spider mite (Tetranychus urticae Koch) after treatments with four acaricide concentrations: 180 mg/l, i.e. maximum recommended concentration for use in glasshouses against spider mites, 18, 1.8, and 0.18 mg/l, i.e. concentration discriminative for eggs and immatures in preliminary studies which produced 100% mortality of these stages. Quiescent female deutonymphs were treated in the first assay, and young pre-ovipositing females in the second and third, in which exposure lasted 6 h and 20 h, respectively. In the first assay, the 180, 18, and 1.8 mg/l concentrations significantly reduced gross fecundity (61-85%), gross fertility (64-87%) and net fertility (85-94%) of the surviving females. In the second one, only the highest concentration achieved a significant statistical reduction in gross fecundity (52%), gross fertility (67%) and net fertility (84%). In the third assay, fecundity and fertility reduction under the two highest concentrations was 98-99% and 93-98%, whereas it was 50-74% under the 1.8 mg/l concentration, and statistically different from control values. In all three trials, treatments with 180, 18, and 1.8 mg/l concentrations significantly reduced the instantaneous rate of increase. In the third assay, treatments with the two highest concentrations caused population decline. Sublethal activity of the 0.18 mg/l concentration was not found in any assay to be statistically significant. Sublethal effects of spiromesifen and its impact on T. urticae management are discussed.
实验室生物测定评估了螺螨酯在四种杀螨剂浓度下对二斑叶螨（Tetranychus urticae Koch）总产卵量、总繁殖力、净繁殖力和种群增长的影响：180mg/L，即温室中防治叶螨的最大推荐浓度；18、1.8 和 0.18mg/L，即初步研究中区分卵和幼螨的浓度，该浓度对这些阶段的死亡率为 100%。在第一次测定中，处理静止的若虫后期；在第二次和第三次测定中，处理年轻的预产卵期雌性，暴露时间分别为 6 小时和 20 小时。在第一次测定中，180、18 和 1.8mg/L 的浓度显著降低了存活雌虫的总产卵量（61-85%）、总繁殖力（64-87%）和净繁殖力（85-94%）。在第二次测定中，只有最高浓度导致总产卵量（52%）、总繁殖力（67%）和净繁殖力（84%）显著降低。在第三次测定中，前两个最高浓度下的产卵量和繁殖力降低了 98-99%和 93-98%，而 1.8mg/L 浓度下的降低了 50-74%，与对照值统计学差异显著。在所有三次试验中，180、18 和 1.8mg/L 浓度处理均显著降低了瞬时增长率。在第三次测定中，前两个最高浓度处理导致种群下降。在任何试验中，0.18mg/L 浓度的亚致死活性均未发现具有统计学意义。讨论了螺螨酯的亚致死作用及其对二斑叶螨管理的影响。
