Banu Sofia, Bhagwat R M, Kadoo N Y, Lagu M D, Gupta V S
Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.
Genetica. 2010 Feb;138(2):197-210. doi: 10.1007/s10709-009-9410-y. Epub 2009 Sep 27.
To characterize the genetic diversity of present populations of Symplocos laurina, which grow in the montane forests in India, we analyzed the DNA sequences of a nuclear gene. Using the 881 bp sequence of cytosolic Glyceraldehyde-3-phosphate dehydrogenase gene, we detected 24 haplotypes among 195 individuals sampled from 14 populations. Two dominant haplotypes were distributed over the entire range of this species in India and several private haplotypes were found. Low genetic diversity within population, high differentiation, number of population specific haplotypes and deviation from neutral evolution characterized the present populations of S. laurina. An analysis of molecular variance indicated the presence of geographic structure within the haplotype distribution. The occurrence of S. laurina preglaciation in India is the most parsimonious explanation for the current geographic structure observed. The populations are presumably ancient and might have spread across its extant distribution range in India through a recent range expansion event.
为了描述生长在印度山地森林中的山矾（Symplocos laurina）现有种群的遗传多样性，我们分析了一个核基因的DNA序列。利用胞质甘油醛-3-磷酸脱氢酶基因的881 bp序列，我们在从14个种群中采集的195个个体中检测到24种单倍型。两种优势单倍型分布在该物种在印度的整个分布范围内，并且发现了几种特有单倍型。种群内遗传多样性低、分化程度高、种群特异性单倍型数量以及偏离中性进化是山矾现有种群特征。分子方差分析表明单倍型分布存在地理结构。印度山矾在冰川期前的出现是对当前观察到的地理结构最简约的解释。这些种群可能很古老，并且可能通过最近的一次范围扩张事件扩散到了其在印度的现有分布范围。
