Lauricella Robert P, Bouteiller Jean-Claude H, Tuccio Béatrice N
Laboratoire TRACES, JE 2421, Case 541, Université de Provence, Faculté de Saint Jérôme, Av. Escadrille Normandie Niemen, 13397 Marseille Cedex 20, France.
Phys Chem Chem Phys. 2005 Jan 21;7(2):399-404. doi: 10.1039/b413931c.
Since major disagreements exist regarding the kinetics of superoxide trapping by nitrones, the underlying theory of one of the most popular method used in these studies was reinvestigated. It involves a competition between the nitrone of interest and a superoxide scavenger, and implies that the superoxide spontaneous dismutation, the spin adduct decay, and the consumption of the competitor during the course of the experiments are negligible events. Evidences of the importance of these three unduly neglected reactions are given, and errors connected to their omission are estimated. Hence this Stern-Volmer-like method should be avoided in the determination of rate constants for the trapping of superoxide by nitrones.
由于在硝酮捕获超氧化物的动力学方面存在重大分歧，因此对这些研究中最常用方法之一的基础理论进行了重新研究。它涉及目标硝酮与超氧化物清除剂之间的竞争，这意味着在实验过程中，超氧化物的自发歧化、自旋加合物的衰变以及竞争者的消耗都是可以忽略不计的事件。文中给出了这三个被过度忽视的反应的重要性的证据，并估计了因忽略它们而产生的误差。因此，在测定硝酮捕获超氧化物的速率常数时，应避免使用这种类似斯特恩-沃尔默的方法。
