Anal Chem. 2009 Nov 1;81(21):9199-200. doi: 10.1021/ac901782d.
Reoptimization of analytical conditions was performed for a high-performance liquid chromatographic (HPLC) detection system for Cu(I) chelators based on the dequenching of Cu(I)-bathocuproine disulfonate complexes that occurs in the presence of Cu(I) chelators. The revision corrects for emission and excitation wavelengths that were in fact second-order light of the actual optimal wavelengths and for the composition of the postcolumn solution. These revisions resulted in an order of magnitude decrease in detection limits of phytochelatins, a class of cysteine-rich, heavy metal-binding peptides. The revised technique is capable of phytochelatin quantitation at femtomole quantities.
重新优化了基于 Cu(I)-二硫代二氮杂菲配合物猝灭的高效液相色谱（HPLC）检测系统的分析条件，用于检测 Cu(I)螯合剂。Cu(I)螯合剂的存在会导致 Cu(I)-二硫代二氮杂菲配合物猝灭。该修订考虑了发射和激发波长实际上是实际最佳波长的二阶光，以及柱后溶液的组成。这些修订导致植物螯合肽（一类富含半胱氨酸的重金属结合肽）的检测限降低了一个数量级。修订后的技术能够对植物螯合肽进行飞摩尔数量级的定量。
