Hu Jing, Li Zhi-wei, Yang Xiu-jin, Yang Yue-jie, Ma Li
Department of Infectious Diseases, Affiliated Shengjing Hospital of China Medical University, Shenyang 110003, China.
Zhonghua Gan Zang Bing Za Zhi. 2009 Sep;17(9):657-60.
To investigate the effect of IFN alpha on the expressions of Collagen I and TGF beta 1 in hepatic stellate cell activated by PDGF-BB.
Hepatic stellate cells (rHSC-99) treated with IFN alpha of different concentration (0, 0.0125, 0.025, 0.050, 0.100, 0.200, 0.400 ng/ml). The cell viability of HSC was measured by MTT. The levels of Col-I mRNA and TGF beta 1 mRNA were measured by the quantitative reverse-transcription polymerase chain reaction (RT-PCR).
(1) When HSC was exposed in PDGF-BB, the cell viability of HSC (1.35 +/- 0.22) was higher than that of the control group (0.890 +/- 0.12) (F = 16.311, P less than 0.05), indicating that PDGF-BB can promote the cell viability of HSC. When HSC was exposed to both PDGF-BB and different concentration of IFN alpha (0.025, 0.05, 0.1, 0.2, 0.4 ng/ml), the cell viability of HSC (0.840 +/- 0.18, 0.450 +/- 0.15, 0.260 +/- 0.01, 0.330 +/- 0.07, 0.30 +/- 0.06) were lower than that of the control group (0.890 +/- 0.12) (F = 7.430, P less than 0.05), indicating that the cell viability of HSC was inhibited when HSC was exposed to both PDGF-BB and different concentrations of IFN alpha. Furthermore, within the range of 0.025 ng/ml to 0.1 ng/ml, the effect of IFN alpha was dose-dependent. (2). The relative expression values of Col-I mRNA in different groups of (0.05, 0.1, 0.2 ng/ml) IFN alpha +PDGF-BB are (0.940 +/- 0.19, 0.610 +/- 0.12, 0.520 +/- 0.02), which were lower than those in the control group (1.410 +/- 0.01) (F = 127.921, P less than 0.05). The relative expression values of TGF beta 1 mRNA in different groups of (0.05, 0.1, 0.2 ng/ml) IFN alpha +PDGF-BB are (1.180 +/- 0.06, 1.150 +/- 0.10, 1.390 +/- 0.04), again were lower than those in the control group (1.620 +/- 0.12) (F = 82.115, P less than 0.05). These results indicated that the expression of Col-I mRNA and TGF beta 1 mRNA was remarkably inhibited when HSC was exposed in both PDGF-BB and IFN alpha.
The cell viability of HSC and the expression of Col-I mRNA and TGF beta 1 mRNA is remarkably inhibited when HSC is exposed in both PDGF-BB and IFN alpha, and the inhibition is dose-dependent.
研究干扰素α对血小板衍生生长因子-BB（PDGF-BB）激活的肝星状细胞中I型胶原蛋白（Collagen I）和转化生长因子β1（TGF beta 1）表达的影响。
用不同浓度（0、0.0125、0.025、0.050、0.100、0.200、0.400 ng/ml）的干扰素α处理肝星状细胞（rHSC-99）。采用MTT法检测肝星状细胞的活力。通过定量逆转录聚合酶链反应（RT-PCR）检测I型胶原（Col-I）mRNA和转化生长因子β1（TGF beta 1）mRNA的水平。
（1）当肝星状细胞暴露于PDGF-BB时，其细胞活力（1.35±0.22）高于对照组（0.890±0.12）（F = 16.311，P＜0.05），表明PDGF-BB可促进肝星状细胞的活力。当肝星状细胞同时暴露于PDGF-BB和不同浓度的干扰素α（0.025、0.05、0.1、0.2、0.4 ng/ml）时，肝星状细胞的活力（0.840±0.18、0.450±0.15、0.260±0.01、0.330±0.07、0.30±0.06）低于对照组（0.890±0.12）（F = 7.430，P＜0.05），表明当肝星状细胞同时暴露于PDGF-BB和不同浓度的干扰素α时，其活力受到抑制。此外，在0.025 ng/ml至0.1 ng/ml范围内，干扰素α的作用呈剂量依赖性。（2）不同组（0.05、0.1、0.2 ng/ml）干扰素α + PDGF-BB中I型胶原（Col-I）mRNA的相对表达值分别为（0.940±0.19、0.610±0.12、0.520±0.02），低于对照组（1.410±0.01）（F = 127.921，P＜0.05）。不同组（0.05、0.1、0.2 ng/ml）干扰素α + PDGF-BB中转化生长因子β1（TGF beta 1）mRNA的相对表达值分别为（1.180±0.06、1.150±0.10、1.390±0.04），同样低于对照组（1.620±0.12）（F = 82.115，P＜0.05）。这些结果表明，当肝星状细胞同时暴露于PDGF-BB和干扰素α时，I型胶原（Col-I）mRNA和转化生长因子β1（TGF beta 1）mRNA的表达明显受到抑制。
当肝星状细胞同时暴露于PDGF-BB和干扰素α时，其细胞活力以及I型胶原（Col-I）mRNA和转化生长因子β1（TGF beta 1）mRNA的表达均受到明显抑制，且这种抑制呈剂量依赖性。
