Xiang Xin, Zhao Wen-bo, Wang Xin
Department of Hematology, Provincial Hospital, Affiliated to Shandong University, Jinan 250021, China.
Zhonghua Gan Zang Bing Za Zhi. 2009 Sep;17(9):661-4.
To study the expression and significance of endothelial cell specific molecule-1 (ESM-1) in hepatocellular carcinoma (HCC) tissue and serum from HCC patients.
The ESM-1 expression was evaluated by immunohistochemistry staining and quantitative real-time PCR in 30 tumor tissues from HCC patients, 15 surrounding non-cancerous hepatic parenchyma, and 15 normal controls. Serum levels of ESM-1 were also measured in the HCC patients and healthy controls by Enzyme-linked immunosorbent assay (ELISA).
ESM-1 was expressed in endothelium of 24 HCC tissues and 6 pericarcinomatous tissues, however, it was not expressed in normal liver tissues. The mRNA level of ESM-1 was 0.064 +/- 0.018, 0.383 +/- 0.103, 0.528 +/- 0.148 in the corresponding tissues, respectively. The mRNA level of ESM-1 in tissues was correlated with the TNM phase of HCC patients, tumor vascular invasion as well as metastasis. The serum ESM-1 was (12.643 +/- 2.280) ng/ml and (4.660 +/- 1.172) ng/ml in hepatic cell carcinoma and normal controls, respectively.
The expression of ESM-1 is significantly up-regulated in HCC, suggesting that ESM-1 may play an important role in the pathoigenesis of HCC.
研究内皮细胞特异性分子-1（ESM-1）在肝细胞癌（HCC）组织及HCC患者血清中的表达及意义。
采用免疫组织化学染色和定量实时PCR检测30例HCC患者肿瘤组织、15例癌旁非癌肝组织及15例正常对照中ESM-1的表达。采用酶联免疫吸附测定（ELISA）法检测HCC患者和健康对照血清中ESM-1水平。
24例HCC组织和6例癌旁组织的内皮细胞中有ESM-1表达，而正常肝组织中未表达。相应组织中ESM-1的mRNA水平分别为0.064±0.018、0.383±0.103、0.528±0.148。组织中ESM-1的mRNA水平与HCC患者的TNM分期、肿瘤血管侵犯及转移相关。肝细胞癌患者和正常对照血清中ESM-1分别为（12.643±2.280）ng/ml和（4.660±1.172）ng/ml。
ESM-1在HCC中表达显著上调，提示ESM-1可能在HCC发病机制中起重要作用。
