抗幽门螺杆菌尿素酶抗体与动脉粥样硬化性疾病有关吗？
Are anti-Helicobacter pylori urease antibodies involved in atherosclerotic diseases?
机构信息
Department of Microbiology, Institute of Biology, Jan Kochanowski University, ul Swietokrzyska 15, 25-406 Kielce, Poland.
出版信息
Clin Biochem. 2010 Jan;43(1-2):115-23. doi: 10.1016/j.clinbiochem.2009.09.016. Epub 2009 Sep 26.
OBJECTIVES
The ureB subunit of urease is a major target recognized by the antibodies of Helicobacter pylori-infected patients. The minimal epitope was determined to be an 8-mer peptide (H-SIKEDVQF-OH).
DESIGN AND METHODS
The aim of this study was to discover whether this synthetic 8-mer peptide (BK-61A) directly recognizes the anti-ureB subunit antibodies of H. pylori-infected and atherosclerotic patients. To achieve a better presentation of the epitopes to antibodies, a new isocyanuric linker was designed and used for to immobilize the peptides on a cellulose support.
RESULTS
In this study a new peptide synthesis method is presented. Anti-ureB antibodies were evaluated by the dot blot technique in 26 H. pylori-infected donors and the sera of 20 H. pylori-infected patients with atherosclerosis using the 8-mer peptide.
CONCLUSIONS
The results reveal that the BK-61A peptide could be used for diagnosing the presence of anti-ureB antibodies that may be involved in the initiation of atherosclerosis.
目的
脲酶的 ureB 亚基是被幽门螺杆菌感染患者的抗体识别的主要靶标。最小表位被确定为一个 8 肽（H-SIKEDVQF-OH）。
设计和方法
本研究的目的是发现这种合成的 8 肽（BK-61A）是否直接识别幽门螺杆菌感染和动脉粥样硬化患者的抗 ureB 亚基抗体。为了更好地向抗体呈现表位，设计了一种新的异氰酸酯连接子，并将肽固定在纤维素载体上。
结果
本研究提出了一种新的肽合成方法。通过斑点印迹技术，用 8 肽检测了 26 名幽门螺杆菌感染供体和 20 名患有动脉粥样硬化的幽门螺杆菌感染患者的抗 ureB 抗体。
结论
结果表明，BK-61A 肽可用于诊断可能参与动脉粥样硬化发生的抗 ureB 抗体的存在。
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