Genetic Toxicology, Biology, FES Iztacala, Universidad Nacional Autónoma de México, 54090 Tlalnepantla, Estado de México, Mexico.
Food Chem Toxicol. 2010 Jan;48(1):120-8. doi: 10.1016/j.fct.2009.09.027. Epub 2009 Sep 26.
Broccoli (Brassica oleracea var. italica) has been defined as a cancer preventive food. Nevertheless, broccoli contains potentially genotoxic compounds as well. We performed the wing spot test of Drosophila melanogaster in treatments with organically grown broccoli (OGB) and co-treatments with the promutagen urethane (URE), the direct alkylating agent methyl methanesulfonate (MMS) and the carcinogen 4-nitroquinoline-1-oxide (4-NQO) in the standard (ST) and high bioactivation (HB) crosses with inducible and high levels of cytochrome P450s (CYPs), respectively. Larvae of both crosses were chronically fed with OGB or fresh market broccoli (FMB) as a non-organically grown control, added with solvents or mutagens solutions. In both crosses, the OGB added with Tween-ethanol yielded the expected reduction in the genotoxicity spontaneous rate. OGB co-treatments did not affect the URE effect, MMS showed synergy and 4-NQO damage was modulated in both crosses. In contrast, FMB controls produced damage increase; co-treatments modulated URE genotoxicity, diminished MMS damage, and did not change the 4-NQO damage. The high dietary consumption of both types of broccoli and its protective effects in D. melanogaster are discussed.
西兰花（ Brassica oleracea var. italica ）被定义为一种预防癌症的食物。然而，西兰花也含有潜在的遗传毒性化合物。我们在黑腹果蝇的翅斑试验中，用有机种植的西兰花（OGB）进行处理，并与促突变剂尿烷（URE）、直接烷化剂甲磺酸甲酯（MMS）和致癌剂 4-硝基喹啉-1-氧化物（4-NQO）进行共处理，分别在标准（ST）和高生物活化（HB）交叉中，诱导和高水平细胞色素 P450s（CYPs）。两种交叉的幼虫都用 OGB 或新鲜市场的西兰花（FMB）作为非有机种植的对照，添加溶剂或诱变剂溶液进行慢性喂养。在两种交叉中，用吐温-乙醇添加 OGB 产生了预期的自发遗传毒性率降低。OGB 共处理对 URE 效应没有影响，MMS 显示协同作用，4-NQO 损伤在两种交叉中都被调节。相比之下，FMB 对照产生了损伤增加；共处理调节了 URE 的遗传毒性，减少了 MMS 的损伤，并且没有改变 4-NQO 的损伤。讨论了两种类型的西兰花的高膳食摄入及其对黑腹果蝇的保护作用。
