Wang Fangjun, Dong Jing, Ye Mingliang, Wu Ren'an, Zou Hanfa
CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Anal Chim Acta. 2009 Oct 12;652(1-2):324-30. doi: 10.1016/j.aca.2009.06.066. Epub 2009 Jul 4.
Capillary column plays an important role in nano-flow liquid chromatography coupled with tandem mass spectrometry for dealing with the high dynamic range and complexity of protein samples in shotgun proteome analysis. In this study, the integrated monolithic frit into the particulate capillary (IMFPC) column was prepared. By comparing the prepared IMFPC column with conventionally fritless capillary column, smaller size of packing materials could be easily packed into the capillary to achieve higher average peak capacity and proteome coverage. As the monolithic emitter was integrated onto this type of column, the void volume between packing particles and electrospray emitter was eliminated and the electrospray quality was improved. The prepared IMFPC column was applied to proteome analysis of mouse liver extracts, and it was observed that the number of identified proteins and peptides increased 14.9 and 12.9% as well as the peak capacity increased 11.6% by using IMFPC column over conventionally fritless capillary column.
在鸟枪法蛋白质组分析中，毛细管柱在纳流液相色谱与串联质谱联用处理蛋白质样品的高动态范围和复杂性方面发挥着重要作用。在本研究中，制备了集成整体式烧结垫到颗粒毛细管（IMFPC）柱中。通过将制备的IMFPC柱与传统的无烧结垫毛细管柱进行比较，可以轻松地将更小尺寸的填充材料填充到毛细管中，以实现更高的平均峰容量和蛋白质组覆盖率。由于整体式发射器集成到了这种类型的柱上，消除了填充颗粒与电喷雾发射器之间的空体积，并提高了电喷雾质量。将制备的IMFPC柱应用于小鼠肝脏提取物的蛋白质组分析，结果表明，与传统的无烧结垫毛细管柱相比，使用IMFPC柱时鉴定出的蛋白质和肽的数量分别增加了14.9%和12.9%，峰容量增加了11.6%。
