改变化学文库筛选中的培养条件可鉴定分枝杆菌的替代抑制剂。
Modifying culture conditions in chemical library screening identifies alternative inhibitors of mycobacteria.
机构信息
School of Biological Sciences, Victoria University of Wellington, Wellington 6012, New Zealand.
出版信息
Antimicrob Agents Chemother. 2009 Dec;53(12):5279-83. doi: 10.1128/AAC.00803-09. Epub 2009 Sep 28.
Abstract
In this study, application of a dual absorbance/fluorescence assay to a chemical library screen identified several previously unknown inhibitors of mycobacteria. In addition, growth conditions had a significant effect on the activity profile of the library. Some inhibitors such as Se-methylselenocysteine were detected only when screening was performed under nutrient-limited culture conditions as opposed to nutrient-rich culture conditions. We propose that multiple culture condition library screening is required for complete inhibitory profiling and for maximal antimycobacterial compound detection.
摘要
在这项研究中，应用双吸收/荧光测定法对化学文库进行筛选，鉴定出了几种以前未知的分枝杆菌抑制剂。此外，生长条件对文库的活性谱有显著影响。一些抑制剂，如硒代蛋氨酸，只有在营养有限的培养条件下进行筛选时才能被检测到，而不是在营养丰富的培养条件下。我们提出，需要进行多种培养条件的文库筛选，以进行完整的抑制谱分析和最大限度地检测抗分枝杆菌化合物。
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