Chabrier P E, Roubert P, Lonchampt M O, Plas P, Braquet P
Institut Henri Beaufour, Les Ulis, France.
Blood Vessels. 1990;27(2-5):153-61. doi: 10.1159/000158806.
Two atrial natriuretic factor (ANF) receptor subtypes are present in vascular smooth muscle cells: the B receptors (or biologically active) coupled to a guanylate cyclase and the C receptors (clearance) representing 95% of the total number of ANF binding sites but noncoupled to a guanylate cyclase. Using binding experiments with 125I-ANF and measurement of cGMP production stimulated by ANF, we were able to demonstrate that ANF receptors are sensitive to homologous (induced by ANF) and heterologous regulation (induced by angiotensin II, AII) in rat cultured vascular smooth muscle cells. The effect of the two hormones showed marked differences, in their time course, their reversibility and their consequence on guanylate cyclase activity. Although both ANF and AII reduced the total number of ANF binding sites after 18 h, ANF induced a desensitization of the guanylate cyclase whereas AII elicited a potentialization of this system. From these results, we have concluded that in vascular cells B receptors are sensitive to homologous regulation and C receptors are sensitive to heterologous regulation by AII. This also highlights a specific interaction between ANF and AII at the receptor level.
血管平滑肌细胞中存在两种心房利钠因子（ANF）受体亚型：与鸟苷酸环化酶偶联的B受体（或生物活性受体）和C受体（清除受体），C受体占ANF结合位点总数的95%，但不与鸟苷酸环化酶偶联。通过使用125I-ANF进行结合实验以及测量ANF刺激产生的环鸟苷酸（cGMP），我们能够证明在大鼠培养的血管平滑肌细胞中，ANF受体对同源调节（由ANF诱导）和异源调节（由血管紧张素II，AII诱导）敏感。这两种激素的作用在时间进程、可逆性以及对鸟苷酸环化酶活性的影响方面表现出显著差异。尽管ANF和AII在18小时后均减少了ANF结合位点的总数，但ANF诱导鸟苷酸环化酶脱敏，而AII则引起该系统的增强作用。从这些结果中，我们得出结论，在血管细胞中，B受体对同源调节敏感，C受体对AII的异源调节敏感。这也突出了ANF和AII在受体水平上的特异性相互作用。
