Schröder Christine, Schumacher Udo, Fogel Mina, Feuerhake Friedrich, Müller Volkmar, Wirtz Ralph M, Altevogt Peter, Krenkel Sylke, Jänicke Fritz, Milde-Langosch Karin
Department of Gynecology, University Medical Center Hamburg-Eppendorf, D-20246 Hamburg, Germany.
Oncol Rep. 2009 Nov;22(5):1109-17. doi: 10.3892/or_00000543.
The L1 adhesion molecule (L1-CAM) is associated with impaired prognosis in many carcinomas. However, limited information about its expression in breast cancer tissue is available. Therefore, we carried out an analysis on L1 expression in primary breast cancers using a combination of Western blot, DNA-microarray analysis and immunohistochemistry. We observed L1 protein and mRNA overexpression in 14-15% of the carcinomas and this was confirmed by immunohistochemical staining. High L1 expression was associated with nodal involvement, high grading, human epidermal growth receptor 2 (Her-2), plasminogen activator inhibitor 1 (PAI-1) and vascular endothelial growth factor (VEGF) expression and a negative estrogen receptor (ER) status, but not with neuroendocrine markers. Moreover, patients with tumors showing high L1-CAM expression had a shorter disease-free and overall survival. Given the emerging functional role of L1 in promoting tumor cell migration, invasion, tumor growth and metastasis, our results suggest that L1 may have this function in breast cancer as well.
L1黏附分子（L1-CAM）与许多癌症的预后不良有关。然而，关于其在乳腺癌组织中表达的信息有限。因此，我们结合蛋白质免疫印迹、DNA微阵列分析和免疫组织化学对原发性乳腺癌中的L1表达进行了分析。我们在14%-15%的癌组织中观察到L1蛋白和mRNA的过表达，免疫组织化学染色证实了这一点。L1高表达与淋巴结受累、高分级、人表皮生长因子受体2（Her-2）、纤溶酶原激活物抑制剂1（PAI-1）和血管内皮生长因子（VEGF）表达以及雌激素受体（ER）阴性状态相关，但与神经内分泌标志物无关。此外，肿瘤显示L1-CAM高表达的患者无病生存期和总生存期较短。鉴于L1在促进肿瘤细胞迁移、侵袭、肿瘤生长和转移方面新出现的功能作用，我们的结果表明L1在乳腺癌中可能也具有这种功能。
