树突状细胞负载 GST-EGFR 融合蛋白：对头颈部鳞癌抗肿瘤免疫的影响。
Dendritic cells pulsed with GST-EGFR fusion protein: effect in antitumor immunity against head and neck squamous cell carcinoma.
机构信息
Department of Otolaryngology, 2nd Affiliated Hospital, College of Medicine, Zhejiang University, Zhejiang, China.
出版信息
Head Neck. 2010 May;32(5):626-35. doi: 10.1002/hed.21233.
BACKGROUND
Overexpression of epidermal growth factor receptor (EGFR) is common in head and neck squamous cell carcinoma (HNSCC). Targeting EGFR is an effective approach to treat EGFR-positive HNSCC. However, the clinical benefits of the present EGFR-targeting agents are still limited in HNSCC patients.
METHODS
Recombinant glutathione-S-transferase (GST)-EGFR fusion protein was produced and purified. Dendritic cells (DCs) of C3H mice were pulsed with fusion protein. Mice were challenged with HNSCC cells before or after vaccination with these DCs, and the cytotoxic T-lymphocyte (CTL) response, interferon-gamma (IFN-gamma) secretion, tumor growth, and survival of mice were assessed.
RESULTS
Significant in vitro and in vivo antitumor activities were observed for mice immunized with DCs pulsed with GST-EGFR fusion protein, compared with the control groups (p < .05).
CONCLUSION
The DCs pulsed with GST-EGFR fusion protein can provide not only preventive but also therapeutic antitumor activities against HNSCC in the animal model.
背景
表皮生长因子受体（EGFR）在头颈部鳞状细胞癌（HNSCC）中过度表达。针对 EGFR 是治疗 EGFR 阳性 HNSCC 的有效方法。然而，目前 EGFR 靶向药物在 HNSCC 患者中的临床获益仍然有限。
方法
产生并纯化重组谷胱甘肽 S-转移酶（GST）-EGFR 融合蛋白。用融合蛋白冲击 C3H 小鼠的树突状细胞（DC）。在接种这些 DC 之前或之后，用 HNSCC 细胞对小鼠进行攻击，并评估 CTL 反应、干扰素-γ（IFN-γ）分泌、肿瘤生长和小鼠的存活情况。
结果
与对照组相比，用 GST-EGFR 融合蛋白冲击的 DC 免疫的小鼠表现出显著的体外和体内抗肿瘤活性（p<0.05）。
结论
用 GST-EGFR 融合蛋白冲击的 DC 不仅可以提供针对 HNSCC 的预防性抗肿瘤活性，还可以提供治疗性抗肿瘤活性。
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