Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, 608 002 Tamil Nadu, India.
Mol Cell Biochem. 2010 Feb;335(1-2):249-54. doi: 10.1007/s11010-009-0274-3. Epub 2009 Sep 30.
Diabetes and smoking have been considered as major health problems individually and their seriousness related to health hazard has been well reported. Data regarding the possible contribution of cigarette smoking to the development of diabetes are scarce and inconclusive. The aim was to investigate the effect of nicotine on diabetes and to analyze the effect of bis demethoxy curcumin analog (BDMCA) in streptozotocin (STZ) and nicotine-induced toxicity. The tissue lipids were extracted according to the method of Folch et al. Plasma and tissue cholesterol was estimated by the method of Allain et al. using reagent kit. Triglycerides were estimated by the method of Foster and Dunn. Free fatty acids were estimated by the method of Falholt et al. Tissue phospholipids were estimated by the method of Zilversmit and Davis. From our study, we found that nicotine not only aggravates diabetic complications but also increased the risk for diabetes. BDMCA, at a dose 80 mg/kg body weight was found to be effective in decreasing toxic effects induced by nicotine and STZ. Our data provide new evidence that cigarette smoking is an additional important factor that could be targeted for the prevention of diabetic complications.
糖尿病和吸烟被认为是单独的主要健康问题，它们对健康危害的严重性已得到充分报道。关于吸烟可能导致糖尿病的发展的数据很少且不确定。目的是研究尼古丁对糖尿病的影响，并分析双去甲氧基姜黄素类似物（BDMCA）在链脲佐菌素（STZ）和尼古丁诱导的毒性中的作用。根据 Folch 等人的方法提取组织脂质。使用 Allain 等人的方法使用试剂盒估计血浆和组织胆固醇。使用 Foster 和 Dunn 的方法估计甘油三酯。使用 Falholt 等人的方法估计游离脂肪酸。使用 Zilversmit 和 Davis 的方法估计组织磷脂。从我们的研究中，我们发现尼古丁不仅加重糖尿病并发症，而且增加患糖尿病的风险。在 80mg/kg 体重的剂量下，BDMCA 被发现可有效降低尼古丁和 STZ 诱导的毒性作用。我们的数据提供了新的证据，表明吸烟是另一个可针对预防糖尿病并发症的重要因素。
