Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Planta Med. 2010 Mar;76(4):402-5. doi: 10.1055/s-0029-1186164. Epub 2009 Sep 29.
The tropane alkaloid anisodamine ( 2) is obtained by 6 beta-hydroxylation of hyoscyamine ( 1). The application of this alkaloid in medicine is gaining attention due to the wide range of therapeutic applications described in addition to its anticholinergic activity. In this work, the production of anisodamine ( 2) by IN VITRO cultures of BRUGMANSIA CANDIDA (Argentinean and Colombian samples) was studied. This alkaloid was estimated in different organs of IN VITRO-germinated seedlings as well as in hairy roots obtained from seedlings from both sources. Colombian roots exhibited the highest content of tropane alkaloids, with anisodamine ( 2) being the main alkaloid measured. In the leaves, the main alkaloid was scopolamine ( 3) and no significant differences were observed between Argentinean and Colombian leaves. The tropane alkaloid content in Argentinean hairy roots was significantly higher than in Colombian ones. Also, in the Argentinean samples the main alkaloid detected was anisodamine ( 2). Argentinean and Colombian B. CANDIDA seedlings and hairy roots appear to be a promising system for the production of anisodamine ( 2).
莨菪烷生物碱山莨菪碱（2）是通过 6β-羟基化莨菪碱（1）得到的。由于其除了具有抗胆碱能活性之外，还具有广泛的治疗应用，因此这种生物碱在医学中的应用越来越受到关注。在这项工作中，研究了 BRUGMANSIA CANDIDA（阿根廷和哥伦比亚样本）的 IN VITRO 培养物生产山莨菪碱（2）的情况。在 IN VITRO 萌发的幼苗的不同器官中以及从两个来源的幼苗获得的发根中均估算了这种生物碱。哥伦比亚根表现出最高的托烷生物碱含量，其中山莨菪碱（2）是测定的主要生物碱。在叶子中，主要的生物碱是东莨菪碱（3），并且在阿根廷和哥伦比亚的叶子之间没有观察到显着差异。阿根廷发根中的托烷生物碱含量明显高于哥伦比亚发根。此外，在阿根廷样品中，检测到的主要生物碱是山莨菪碱（2）。阿根廷和哥伦比亚的 B. CANDIDA 幼苗和发根似乎是生产山莨菪碱（2）的有前途的系统。
