Divison of Radiation Medicine, Jilin University School of Public Health, Changchun, China.
Environ Mol Mutagen. 2010 Apr;51(3):215-21. doi: 10.1002/em.20523.
Micronucleated reticulocyte (MN-RET) scoring by flow cytometry (FCM) has been used in assessment of the clastogenic effects of chemicals. However, its dose-response to acute whole body irradiation (WBI) at moderate dose rates remains to be established. We show that FCM scoring of MN-RET in peripheral blood from male ICR mice exposed to WBI X-ray doses of 0.5-5 Gy at a dose rate of 0.488 Gy/min exhibits a linear dose-response relationship 24, 48, and 72 hr following WBI. Parallel microscopic counting of micronucleated polychromatic erythrocytes (MN-PCE) in bone marrow smears from the same animals showed similar linear dose-response patterns at the same time points. Indeed, MN-RET and MN-PCE were highly correlated at all doses and time points. In view of the speed and accuracy of this method, in addition to the small blood sample size needed for the assay, the flow cytometric protocol for MN-RET scoring may provide a minimally-invasive, high throughput radiation biodosimeter.
流式细胞术（FCM）检测有核红细胞微核（MN-RET）评分已用于评估化学物质的致裂作用。然而，其对中剂量率急性全身照射（WBI）的剂量反应仍有待确定。我们表明，雄性 ICR 小鼠外周血中 MN-RET 的 FCM 评分在 WBI X 射线剂量为 0.5-5 Gy、剂量率为 0.488 Gy/min 时，在 WBI 后 24、48 和 72 小时呈现出线性剂量反应关系。来自同一动物的骨髓涂片上微核多色红细胞（MN-PCE）的平行显微镜计数在相同时间点显示出相似的线性剂量反应模式。事实上，MN-RET 和 MN-PCE 在所有剂量和时间点均高度相关。鉴于该方法的速度和准确性，以及该测定所需的小样本量，MN-RET 评分的流式细胞术方案可能提供一种微创、高通量的辐射生物剂量计。
