Orita M, Sekiya T, Hayashi K
Oncogene Division, National Cancer Center Research Institute, Tokyo, Japan.
Genomics. 1990 Oct;8(2):271-8. doi: 10.1016/0888-7543(90)90282-y.
We have developed an efficient method for detection of sequence differences in genomic DNA based on a new principle (M. Orita et al., 1989, Genomics 5: 874-879). Using this method, we show here that approximately half the Alu repeats interspersed in the human genome are significantly polymorphic. Analysis of Alu repeat polymorphism should be useful in construction of a high-resolution map and also in identifying genotypes of individuals for clinical and other purposes because the repeats are ubiquitous and the technique for their detection is simple.
我们基于一项新原理（M. 大rita等人，1989年，《基因组学》5：874 - 879）开发了一种用于检测基因组DNA序列差异的高效方法。利用该方法，我们在此表明，散布于人类基因组中的约一半Alu重复序列具有显著的多态性。对Alu重复序列多态性的分析在构建高分辨率图谱以及鉴定个体基因型以用于临床和其他目的方面应会很有用，因为这些重复序列无处不在且检测它们的技术很简单。
