Hassall C J, Penketh R, Rodeck C, Burnstock G
Department of Anatomy and Developmental Biology, University College, London, United Kingdom.
Anat Embryol (Berl). 1990;182(4):329-37. doi: 10.1007/BF02433493.
Dissociated cell culture preparations were employed to study intracardiac neurones of the atria from human fetal hearts at 9 to 21 weeks' gestation. Intracardiac neurones were not observed in cultures dissociated from the ventricles. Single neurones, as well as groups, could be identified by phase-contrast microscopy in all of the atrial cultures prepared from 14 to 21 weeks' gestation, and protein gene product 9.5-like immunoreactive neurones were detected in cultures from as early as 10 weeks' gestation. The neurones were mononucleate, with a prominent nucleolus or multiple nucleoli, and often had extensive neurites. Neurones tended to be bigger in cultures from later stages in gestation, and these cells appeared to be more mature with a complex pattern of neurite outgrowth. Many neurones from 15 to 20 weeks' gestation expressed somatostatin-like immunoreactivity in culture. A very low proportion of cultured neurones was immunoreactive for neuropeptide Y and its C-terminal flanking peptide. Neuropeptide Y-like immunoreactive neurones also contained 5-hydroxy-tryptamine-like immunoreactivity in culture, but dopamine beta-hydroxylase-like immunoreactive neurones were not detected. This study is the first description of human intracardiac neurones in culture and forms the essential baseline for further direct investigation of these cells.
采用解离细胞培养制剂来研究妊娠9至21周的人类胎儿心脏心房内的神经元。在从心室解离的培养物中未观察到心内神经元。在所有从妊娠14至21周制备的心房培养物中，通过相差显微镜可以识别单个神经元以及神经元群，并且早在妊娠10周的培养物中就检测到了蛋白基因产物9.5样免疫反应性神经元。这些神经元为单核，具有明显的核仁或多个核仁，并且通常具有广泛的神经突。来自妊娠后期培养物中的神经元往往更大，并且这些细胞似乎更成熟，具有复杂的神经突生长模式。许多来自妊娠15至20周的神经元在培养物中表达生长抑素样免疫反应性。培养的神经元中对神经肽Y及其C末端侧翼肽呈免疫反应性的比例非常低。神经肽Y样免疫反应性神经元在培养物中也含有5-羟色胺样免疫反应性，但未检测到多巴胺β-羟化酶样免疫反应性神经元。本研究首次描述了培养中的人类心内神经元，并为进一步直接研究这些细胞奠定了重要的基础。
