Kauc L, Mitchell M A, Goodgal S H
Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104.
Gene. 1990 Oct 30;95(1):149-53. doi: 10.1016/0378-1119(90)90427-s.
Previous analysis of physical maps of Haemophilus influenzae, which is circular and 1.9 Mb in length [Lee and Smith, J. Bacteriol. 170 (1988) 4402-4405; Kauc et al., J. Bacteriol. 171 (1989) 2474-2479], did not detect any NotI (GCGGCCGC) restriction sites. A transposon, Tn916, was constructed to contain a NotI linker cloned into its NciI site and introduced into the H. influenzae chromosome. NotI digestion of chromosomes containing a Tn916-associated NotI site followed by separation of fragments by field-inversion gel electrophoresis revealed the presence of two fragments obtained by two NotI cuts, one in Tn916 and the other, a unique, 'natural' NotI site in the original chromosomal DNA. The examination of other Haemophilus strains demonstrated the presence of one or more NotI sites in all of those tested.
先前对流感嗜血杆菌物理图谱的分析表明，其基因组呈环状，长度为1.9 Mb[Lee和Smith，《细菌学杂志》170(1988)4402 - 4405；Kauc等人，《细菌学杂志》171(1989)2474 - 2479]，未检测到任何NotI（GCGGCCGC）限制酶切位点。构建了一个转座子Tn916，使其在NciI位点克隆有一个NotI接头，并将其导入流感嗜血杆菌染色体。对含有与Tn916相关的NotI位点的染色体进行NotI酶切，然后通过场反转凝胶电泳分离片段，结果显示存在两个由两次NotI切割获得的片段，一个在Tn916中，另一个是原始染色体DNA中独特的“天然”NotI位点。对其他流感嗜血杆菌菌株的检测表明，在所有测试菌株中都存在一个或多个NotI位点。
