Department of Biomedical Engineering, State University of New York-Stony Brook, Stony Brook, NY 11794-8181, USA.
J Biomater Sci Polym Ed. 2009;20(13):1915-28. doi: 10.1163/156856208X393202.
A series of in situ gelable hydrogels has been prepared from oxidized alginate (Oalg) and teleostean. The gelation process is monitored rheologically; the effects of gelling temperature, oxidation degree of alginate and weight ratio of Oalg/teleostean on gelation are investigated. The results show that the Oalg/teleostean system solidifies quickly at body temperature. Moreover, the structure of the hydrogels is highly porous, the pore sizes, as well as swelling and degradation, can be controlled by varying the cross-linking density. Both gelation time and mechanical properties of the Oalg/teleostean system can be modulated by adjusting the oxidation degree of alginate and the weight ratio of Oalg/teleostean. The potential toxicity of the hydrogels are determined by long-term in vitro viability tests and the results reveal that the hydrogels formulations are non-cytotoxic; in parallel, the Oalg/teleostean hydrogel is amenable to cell attachment, proliferation and migration.
已制备了一系列可原位凝胶化的氧化海藻酸盐（Oalg）和硬骨鱼类水凝胶。通过流变学监测凝胶化过程；研究了凝胶化温度、海藻酸钠的氧化程度和 Oalg/硬骨鱼类的重量比对凝胶化的影响。结果表明，Oalg/硬骨鱼类体系在体温下快速凝固。此外，水凝胶的结构为高度多孔结构，通过改变交联密度可以控制孔径大小以及溶胀和降解。通过调整海藻酸钠的氧化程度和 Oalg/硬骨鱼类的重量比，可以调节 Oalg/硬骨鱼类体系的凝胶时间和机械性能。通过长期体外生存能力测试来确定水凝胶的潜在毒性，结果表明水凝胶配方无细胞毒性；同时，Oalg/硬骨鱼类水凝胶适合细胞附着、增殖和迁移。
