Wood J N, Bevan S J, Coote P R, Dunn P M, Harmar A, Hogan P, Latchman D S, Morrison C, Rougon G, Theveniau M
Sandoz Institute for Medical Research, London, U.K.
Proc Biol Sci. 1990 Sep 22;241(1302):187-94. doi: 10.1098/rspb.1990.0084.
Hybrid cell lines derived from neonatal rat dorsal root ganglia neurons fused with the mouse neuroblastoma N18Tg2 exhibit sensory neuron-like properties not displayed by the parental neuroblastoma. These properties include an inward (depolarizing) current with a conductance increase in response to activation of a bradykinin receptor, an inward (depolarizing) current with a conductance increase in response to the sensory excitotoxin capsaicin, the expression of sensory neuropeptides (substance P, CGRP and somatostatin), the expression of phosphatidylinositol-anchored molecules including adhesion molecules of the immunoglobulin superfamily that can be regulated in serum-free culture by nerve growth factor (N-CAM, F-3 and Thy-1), and low permissivity to herpes simplex virus infection. These lines thus provide appropriate models for the study of mechanisms involved in nociceptor activation and the regulation of expression of sensory-neuron specific markers including neuropeptides.
源自新生大鼠背根神经节神经元与小鼠神经母细胞瘤N18Tg2融合的杂交细胞系表现出亲代神经母细胞瘤所不具备的感觉神经元样特性。这些特性包括：一种内向（去极化）电流，其电导随着缓激肽受体激活而增加；一种内向（去极化）电流，其电导随着感觉兴奋性毒素辣椒素激活而增加；感觉神经肽（P物质、降钙素基因相关肽和生长抑素）的表达；磷脂酰肌醇锚定分子的表达，包括免疫球蛋白超家族的黏附分子，这些分子在无血清培养中可被神经生长因子（神经细胞黏附分子、F-3和Thy-1）调控；以及对单纯疱疹病毒感染的低易感性。因此，这些细胞系为研究伤害感受器激活机制以及包括神经肽在内的感觉神经元特异性标志物表达调控提供了合适的模型。
