Department of Pathology and Biodefense University Hospital; Faculty of Medicine; Saga University; Nabeshima, Saga Japan.
Organogenesis. 2009 Apr;5(2):50-6. doi: 10.4161/org.5.2.8347.
Adipose tissue consists of mature adipocytes, preadipocytes and mesenchymal stem cells (MSCs), but a culture system for analyzing their cell types within the tissue has not been established. We have recently developed "adipose tissue-organotypic culture system" that maintains unilocular structure, proliferative ability and functions of mature adipocytes for a long term, using three-dimensional collagen gel culture of the tissue fragments. In this system, both preadipocytes and MSCs regenerate actively at the peripheral zone of the fragments. Our method will open up a new way for studying both multiple cell types within adipose tissue and the cell-based mechanisms of obesity and metabolic syndrome. Thus, it seems to be a promising model for investigating adipose tissue biology and regeneration. In this article, we introduce adipose tissue-organotypic culture, and propose two theories regarding the mechanism of tissue regeneration that occurs specifically at peripheral zone of tissue fragments in vitro.
脂肪组织由成熟脂肪细胞、前体细胞和间充质干细胞（MSCs）组成，但尚未建立用于分析组织内这些细胞类型的培养系统。我们最近开发了“脂肪组织器官型培养系统”，该系统使用组织碎片的三维胶原凝胶培养，长期维持单室结构、成熟脂肪细胞的增殖能力和功能。在该系统中，前体细胞和 MSCs 在外周区域积极再生。我们的方法将为研究脂肪组织内的多种细胞类型以及肥胖和代谢综合征的基于细胞的机制开辟新的途径。因此，它似乎是研究脂肪组织生物学和再生的有前途的模型。在本文中，我们介绍了脂肪组织器官型培养，并提出了两种关于组织再生机制的理论，该机制在体外组织碎片的外周区域特异性发生。
