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[水杨酸对NaCl胁迫下番茄幼苗蔗糖代谢的影响]

[Effects of salicylic acid on sucrose metabolism of tomato seedlings under NaCl stress].

作者信息

Su Yue, Li Tian-Lai, Li Nan, Yang Feng-Jun, Lu Shao-Wei

机构信息

Liaoning Provincial Key Laboratory of Protected Horticulture, College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2009 Jun;20(6):1525-8.

Abstract

A water culture experiment was conducted with the seedlings of tomato (Lycopersicon esculentum Mill.) cultivar Liaoyuanduoli to study the effects of salicylic acid (SA) on their sucrose metabolism under NaCl stress. The seedlings were treated with different concentrations (100, 300, and 500 mg x L(-1)) of SA, and the contents of sucrose, glucose, and fructose as well as the related enzyme activities of sucrose metabolism, including acid invertase (AI), neutral invertase (NI), sucrose phosphate synthase (SPS), and sucrose synthase (SS), in seedling leaves were determined. Under NaCl stress, SA could maintain or enhance the leaf fructose and glucose contents and the leaf AI, NI, SPS and SS activities, with the highest increment of fructose and glucose contents being 30.0% and 31.1% and that of AI, NI, SPS and SS activities being 24.7%, 27.9%, 22.0% and 24.5%, respectively, in comparing with no SA application, while had less effect on the leaf sucrose content, which suggested that SA could play a protective role in the NaCl-tolerance of tomato seedlings via enhancing the leaf invertase activity to increase leaf fructose and glucose contents. The best alleviating effect was observed at 500 mg x L(-1) of SA.

摘要

以番茄(Lycopersicon esculentum Mill.)品种辽园多丽的幼苗为材料进行水培试验,研究水杨酸(SA)对NaCl胁迫下番茄幼苗蔗糖代谢的影响。用不同浓度(100、300和500 mg·L⁻¹)的SA处理幼苗,测定幼苗叶片中蔗糖、葡萄糖和果糖的含量以及蔗糖代谢相关酶活性,包括酸性转化酶(AI)、中性转化酶(NI)、蔗糖磷酸合成酶(SPS)和蔗糖合酶(SS)。在NaCl胁迫下,SA能维持或提高叶片果糖和葡萄糖含量以及叶片AI、NI、SPS和SS活性,与不施用SA相比,果糖和葡萄糖含量的最高增幅分别为30.0%和31.1%,AI、NI、SPS和SS活性的最高增幅分别为24.7%、27.9%、22.0%和24.5%,而对叶片蔗糖含量影响较小,这表明SA可通过提高叶片转化酶活性增加叶片果糖和葡萄糖含量,从而对番茄幼苗的耐NaCl性起到保护作用。在SA浓度为500 mg·L⁻¹时观察到最佳缓解效果。

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