Medicine University of Tunis, Tunis, Tunisia.
Clin Exp Rheumatol. 2009 Mar-Apr;27(2 Suppl 53):S58-62.
The manifestations of BD are considered to have developed as a result of immunological dysfunction, which is suggested to be induced by microbial pathogens. The Toll-like receptor (TLR) genes were known to be associated with a variety of infectious diseases due to their central role in both innate and adaptive immunity. In this report, we investigated the possible association between BD patients and genetic variations within the TLR 2, 4 and 9 genes in a Tunisian population.
135 Tunisian BD patients and 159 healthy blood donors from the same geographical area were genotyped by polymerase chain reaction for the TLR polymorphisms.
Among the TLR polymorphisms, only the distribution of TLR9 1486 T/C genotype (p=0.07; chi2=3.30; OR=1.54; 95% CI=0.94-2.51) and allele (p=0.08; chi2=2.91; OR=1.34; 95% CI=0.94-1.92) frequencies was different between BD patients and healthy controls, but did not reach statistical significance. For the TLR9 1237 T/C, the distribution of genotypes and alleles were not significantly different comparing total patients with controls. There were no associations between the studied polymorphisms and the main clinical manifestations of BD. The G, T and A allele of the TLR4 1896 A/G, TLR4 11196 C/T and TLR2 12408 G/A polymorphisms were uncommon and absent in the Tunisian population.
Our results showed that SNPs in the TLR2, 4 and 9 genes were not significantly associated with susceptibility to BD.
BD 的表现被认为是由于免疫功能障碍而发展的，这被认为是由微生物病原体引起的。Toll 样受体 (TLR) 基因由于在先天和适应性免疫中都具有核心作用，因此被认为与多种传染病有关。在本报告中，我们在突尼斯人群中研究了 TLR2、4 和 9 基因中的遗传变异与 BD 患者之间的可能关联。
通过聚合酶链反应对来自同一地理区域的 135 名突尼斯 BD 患者和 159 名健康献血者进行 TLR 多态性基因分型。
在 TLR 多态性中，只有 TLR9 1486 T/C 基因型（p=0.07；chi2=3.30；OR=1.54；95%CI=0.94-2.51）和等位基因（p=0.08；chi2=2.91；OR=1.34；95%CI=0.94-1.92）的分布在 BD 患者和健康对照组之间不同，但没有达到统计学意义。对于 TLR9 1237 T/C，总患者与对照组的基因型和等位基因分布没有显着差异。在所研究的多态性与 BD 的主要临床表现之间没有关联。TLR4 1896 A/G、TLR4 11196 C/T 和 TLR2 12408 G/A 多态性的 G、T 和 A 等位基因在突尼斯人群中罕见且不存在。
我们的结果表明，TLR2、4 和 9 基因中的 SNP 与 BD 的易感性没有显着关联。
