TLR4 多态性可作为中国汉族儿童特应性皮炎的潜在预测指标。
TLR4 polymorphisms as potential predictors of atopic dermatitis in Chinese Han children.
机构信息
Department of Clinical Laboratory, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center For Child Health, Hangzhou, China.
Zhejiang University School of Medicine, Hangzhou, China.
出版信息
J Clin Lab Anal. 2022 May;36(5):e24385. doi: 10.1002/jcla.24385. Epub 2022 Mar 29.
BACKGROUND
Toll-like receptor 4 (TLR4) is considered to be involved in the pathogenesis and progression of atopic dermatitis (AD). In the present study, we evaluated the relationship between TLR4 gene polymorphisms and the susceptibility or severity of AD among Chinese Han children.
METHODS
A total of 132 AD patients and 100 healthy controls were enrolled in this study. Four single-nucleotide polymorphisms (rs19277914, rs11536891, rs7869402, and rs11536889) of the TLR4 gene were genotyped by multiplex PCR combined with next-generation sequencing.
RESULTS
Our results showed that a significantly reduced risk for AD was associated with C allele [p = 0.008; odds ratio (OR) = 0.41, C vs. T], TC genotype (p = 0.022; OR = 0.41, TC vs. TT), and TC + CC genotype (p = 0.010; OR = 0.39, TC + CC vs. TT) of TLR4 rs11536891. The frequency of the haplotype GCCG (rs1927914-rs11536891-rs7869402-rs11536889) in AD patients was lower than that in the controls (p = 0.010; OR = 0.38). Moreover, the results indicated that a higher risk of severe AD was related to the T allele (p = 0.019; OR = 2.97, T vs. C) and the TC genotype (p = 0.021; OR = 3.34, TC vs. CC) of TLR4 rs7869402. A risk haplotype of TLR4 (GTTG) was found in severe AD patients (p = 0.010; OR = 5.26).
CONCLUSIONS
Our data suggested that TLR4 rs11536891 polymorphism was associated with the susceptibility to AD in Chinese Han children. And TLR4 rs7869402 might confer the severity of pediatric AD patients.
背景
Toll 样受体 4（TLR4）被认为参与了特应性皮炎（AD）的发病机制和进展。在本研究中，我们评估了 TLR4 基因多态性与中国汉族儿童 AD 的易感性或严重程度之间的关系。
方法
本研究纳入了 132 例 AD 患者和 100 例健康对照者。采用多重 PCR 结合下一代测序技术对 TLR4 基因的 4 个单核苷酸多态性（rs19277914、rs11536891、rs7869402 和 rs11536889）进行基因分型。
结果
研究结果显示，与 T 等位基因（p=0.008；OR=0.41，C 与 T）、TC 基因型（p=0.022；OR=0.41，TC 与 TT）和 TC+CC 基因型（p=0.010；OR=0.39，TC+CC 与 TT）相比，TLR4 rs11536891 的 C 等位基因显著降低了 AD 的发病风险。AD 患者中 TLR4 rs1927914-rs11536891-rs7869402-rs11536889 单体型 GCCG 的频率低于对照组（p=0.010；OR=0.38）。此外，结果表明，TLR4 rs7869402 的 T 等位基因（p=0.019；OR=2.97，T 与 C）和 TC 基因型（p=0.021；OR=3.34，TC 与 CC）与严重 AD 风险相关。在严重 AD 患者中发现了 TLR4（GTTG）的风险单体型（p=0.010；OR=5.26）。
结论
本研究数据表明，TLR4 rs11536891 多态性与中国汉族儿童 AD 的易感性相关，而 TLR4 rs7869402 可能与儿科 AD 患者的严重程度相关。
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