Fuse Y, Polk D H, Lam R W, Reviczky A L, Fisher D A
Department of Pediatrics, Harbor-University of California Los Angeles Medical Center, Torrance 90509.
Am J Physiol. 1990 Dec;259(6 Pt 1):E787-91. doi: 10.1152/ajpendo.1990.259.6.E787.
The distribution and ontogeny of tissue prolyl endopeptidase and pyroglutamyl peptidase I activities were studied in the rat from the 7th day before birth to adulthood. While low levels of prolyl endopeptidase activity were demonstrable in many fetal tissues, activity in brain cortex, hypothalamus, lung, and kidney increased dramatically during the 2 wk after birth, gradually returning to adult levels. In adult rats, levels of tissue prolyl endopeptidase activity were highest in kidney, when compared with the intermediate levels in brain cortex, hypothalamus, and liver. Pyroglutamyl peptidase activity was widely distributed in adult rat tissues with high levels in kidney and liver that exceeded intermediate levels in brain cortex and hypothalamus. Pyroglutamyl peptidase activities in fetal gut, brain, and lung tissue were elevated above adult values. In contrast to the development changes in prolyl endopeptidase activities, pyroglutamyl peptidase activity remained elevated above adult levels only during the first week of life. These results indicate that both prolyl endopeptidase and pyroglutamyl peptidase activities in the rat are developmentally regulated.
研究了从出生前7天到成年大鼠组织中脯氨酰内肽酶和焦谷氨酰肽酶I活性的分布及个体发生情况。虽然在许多胎儿组织中可检测到低水平的脯氨酰内肽酶活性，但出生后2周内，大脑皮质、下丘脑、肺和肾脏中的活性显著增加，随后逐渐恢复到成年水平。在成年大鼠中，与大脑皮质、下丘脑和肝脏中的中等水平相比，肾脏中组织脯氨酰内肽酶活性最高。焦谷氨酰肽酶活性在成年大鼠组织中广泛分布，肾脏和肝脏中的水平较高，超过大脑皮质和下丘脑的中等水平。胎儿肠道、大脑和肺组织中的焦谷氨酰肽酶活性高于成年值。与脯氨酰内肽酶活性的发育变化相反，焦谷氨酰肽酶活性仅在出生后第一周高于成年水平。这些结果表明，大鼠体内脯氨酰内肽酶和焦谷氨酰肽酶的活性均受到发育调控。
