Department of Anatomy, School of Basic Medical Sciences, Key Laboratory on Machine Perception, Peking University, Beijing, China.
Invest Ophthalmol Vis Sci. 2010 Feb;51(2):1208-15. doi: 10.1167/iovs.09-4179. Epub 2009 Sep 24.
To evaluate early changes in the visual response properties of Y cells in the detached feline retina.
The retinas of young adult cats were detached by injection, with a glass micropipette, of a solution of 0.004% sodium hyaluronate in a balanced salt solution between the neural retina and the retinal pigment epithelium. At 1, 3, and 7 days after detachment, the eyes were removed. The eyecup was prepared as a flat mount in a recording chamber and superfused with medium. Extracellular single-unit responses from Y cells in the retinas were recorded.
One, 3, and 7 days after retinal detachment surgery, Y cells showed clear signs of functional deterioration. At each time point, more ON center cells than OFF cells were encountered. Y cells in the detached retinas showed a statistically significant elevation in the average threshold irradiance after 1-, 3-, and 7-day detachment, respectively. The average contrast threshold recorded from cells in the normal retina was 3.6%, but it increased to 14.5%, 21.8%, and 47.5% after 1-, 3-, and 7-day detachment, respectively. Furthermore, at each time point, the capability of Y cells to process contrast information decreased significantly more because of detachment than because of luminance task performance.
Retinal detachment induced rapid functional remodeling that resulted in degenerated Y-cell function, including an elevated luminance threshold and a deteriorated contrast threshold. Detachment had a greater impact on the latter. These physiological changes after retinal detachment could be used as objective indicators of early deterioration of visual function in future studies of retinal remodeling.
评估脱离猫视网膜后 Y 细胞视觉反应特性的早期变化。
用玻璃微管将 0.004%透明质酸钠溶液注入视网膜神经层和视网膜色素上皮之间，使年轻成年猫的视网膜脱离。在脱离后 1、3 和 7 天，取出眼球。将眼杯制备为记录室中的平面安装物并进行介质超流。记录视网膜中 Y 细胞的细胞外单细胞反应。
视网膜脱离手术后 1、3 和 7 天，Y 细胞出现明显的功能恶化迹象。在每个时间点，遇到的 ON 中心细胞都多于 OFF 细胞。在脱离的视网膜中，Y 细胞的平均阈值辐照度分别在 1、3 和 7 天脱离后显著升高。从正常视网膜中记录的细胞的平均对比度阈值为 3.6%，但分别增加到 14.5%、21.8%和 47.5%，在 1、3 和 7 天脱离后。此外，在每个时间点，由于脱离而不是由于亮度任务性能，Y 细胞处理对比度信息的能力明显下降。
视网膜脱离引起的快速功能重塑导致 Y 细胞功能退化，包括亮度阈值升高和对比度阈值恶化。脱离对后者的影响更大。这些视网膜脱离后的生理变化可作为未来视网膜重塑研究中视觉功能早期恶化的客观指标。
