β受体激动剂对培养的肌肉细胞的作用。

The effects of beta agonists on muscle cells in culture.

作者信息

Harper J M, Mackinson I, Buttery P J

机构信息

Department of Applied Biochemistry and Food Science, University of Nottingham, Faculty of Agricultural Science, Loughborough, Leics.

出版信息

Domest Anim Endocrinol. 1990 Oct;7(4):477-84. doi: 10.1016/0739-7240(90)90005-k.

DOI:10.1016/0739-7240(90)90005-k

PMID:1979765

Abstract

Increases in protein synthesis of 12% were found with two myogenic cell lines (L6 and G8-1) on treatment for 6 hr with the beta-adrenergic agonist cimaterol. In L6 cells, propranolol blocked the effect. Protein breakdown measured over 18-24 hr was unchanged. The Kd for cimaterol binding to the L6 beta-receptor was 26 nM which was compatible with its EC50 for the stimulation of protein synthesis (approx 5 nM). Evidence provided with muscle cell lines indicates a direct effect of cimaterol on protein synthesis, which may contribute to muscle accretion in cimaterol-fed animals.

摘要

在用β-肾上腺素能激动剂西马特罗处理6小时后，发现两种成肌细胞系（L6和G8-1）的蛋白质合成增加了12%。在L6细胞中，普萘洛尔可阻断该效应。在18至24小时内测得的蛋白质分解未发生变化。西马特罗与L6β受体结合的解离常数（Kd）为26 nM，这与其刺激蛋白质合成的半数有效浓度（EC50，约5 nM）相符。肌肉细胞系提供的证据表明西马特罗对蛋白质合成有直接作用，这可能有助于食用西马特罗的动物增加肌肉量。