Department of Nuclear Medicine, Faculty of Medicine, Kuwait University, 13110 Safat Kuwait.
Med Princ Pract. 2009;18(6):466-9. doi: 10.1159/000235896. Epub 2009 Sep 30.
The objective of this study was to investigate the intracellular localization of technetium 99m-hexamethylpropylene amine oxime ((99m)Tc-d,l-HMPAO) in rat red blood cells (RBCs) and plasma.
Sprague-Dawley rats (n = 36) were injected with 129.5 MBq (99m)Tc-d,l-HMPAO. Using a heparinized syringe, 2-ml blood samples were withdrawn from the rats 10, 20, 30, 40, 50 and 60 min after (99m)Tc-d,l-HMPAO injection. Blood was centrifuged for 10 min at 2,240 rpm to separate RBCs from plasma. A chloroform/methanol/distilled water mixture was added to the RBCs and plasma in separate tubes to extract (99m)Tc-d,l- HMPAO into aqueous (Aq) and lipid (Lp) phases. The (99m)Tc-d,l-HMPAO activities in Aq and Lp phases were measured separately using a dose calibrator. The data were analyzed using Student's t test and a one-way ANOVA.
More than 70% of the injected dose of (99m)Tc-d,l-HMPAO was retained in the RBCs for up to 60 min, compared to less than 20% in plasma. The uptakes in the Aq for plasma were 88.77 +/- 1.04, 94.23 +/- 2.42, 90.15 +/- 1.88, 98.59 +/- 0.89, 96.42 +/- 1.50 and 97.59 +/- 1.92% at 10, 20, 30, 40, 50 and 60 min after injection, respectively. The corresponding uptakes in the Lp phases were: 11.23 +/- 0.04, 5.77 +/- 0.07, 9.85 +/- 0.06, 1.41 +/- 0.08, 3.58 +/- 0.04 and 2.41 +/- 0.06, respectively. Similar values (% +/- SE) and trends were obtained in the Aq and Lp phases for RBCs. A highly significant difference between the Aq and Lp phases at each time point studied was observed (p < 0.0001). There was no statistically significant difference between the time points in the Aq phase, but there was a significant difference (p < 0.001) between time points in the Lp phase.
This study demonstrated that most of the intracellular localization of (99m)Tc-d,l-HMPAO was in the Aq phase compared to Lp phase in both RBCs and plasma up to 60 min.
本研究旨在探讨 99mTc-双半胱乙酯（99mTc-d,l-HMPAO）在大鼠红细胞（RBC）和血浆中的细胞内定位。
36 只 Sprague-Dawley 大鼠注射 129.5MBq 99mTc-d,l-HMPAO。在注射 99mTc-d,l-HMPAO 后 10、20、30、40、50 和 60min，使用肝素化注射器从大鼠体内抽取 2ml 血样。将血液以 2240rpm 的速度离心 10min，以将 RBC 与血浆分离。将氯仿/甲醇/蒸馏水混合物加入到 RBC 和血浆的单独管中，以将 99mTc-d,l-HMPAO 提取到水性（Aq）和脂质（Lp）相中。使用剂量计数器分别测量 Aq 和 Lp 相中 99mTc-d,l-HMPAO 的活性。使用学生 t 检验和单因素方差分析对数据进行分析。
与血浆中的不到 20%相比，在注射后 60min 内，超过 70%的注射剂量的 99mTc-d,l-HMPAO 保留在 RBC 中。在注射后 10、20、30、40、50 和 60min，血浆中 Aq 的摄取率分别为 88.77 +/- 1.04%、94.23 +/- 2.42%、90.15 +/- 1.88%、98.59 +/- 0.89%、96.42 +/- 1.50%和 97.59 +/- 1.92%。相应的 Lp 相摄取率分别为：11.23 +/- 0.04%、5.77 +/- 0.07%、9.85 +/- 0.06%、1.41 +/- 0.08%、3.58 +/- 0.04%和 2.41 +/- 0.06%。在 RBC 中，Aq 和 Lp 相中获得了相似的（% +/- SE）和趋势值。在研究的每个时间点，Aq 和 Lp 相之间均观察到高度显著差异（p < 0.0001）。在 Aq 相中各时间点之间没有统计学上的显著差异，但在 Lp 相中时间点之间有显著差异（p < 0.001）。
本研究表明，在 RBC 和血浆中，与 Lp 相比，99mTc-d,l-HMPAO 的细胞内定位在 60min 内大部分在 Aq 相中。
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