Department of Biochemistry and Molecular Biology and Eppley Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska 68198-5870, USA.
Protein Sci. 2009 Dec;18(12):2471-9. doi: 10.1002/pro.258.
Eps15 homology (EH)-domain containing proteins are regulators of endocytic membrane trafficking. EH-domain binding to proteins containing the tripeptide NPF has been well characterized, but recent studies have shown that EH-domains are also able to interact with ligands containing DPF or GPF motifs. We demonstrate that the three motifs interact in a similar way with the EH-domain of EHD1, with the NPF motif having the highest affinity due to the presence of an intermolecular hydrogen bond. The weaker affinity for the DPF and GPF motifs suggests that if complex formation occurs in vivo, they may require high ligand concentrations, the presence of successive motifs and/or specific flanking residues.
EH 结构域包含蛋白是内吞膜运输的调节剂。EH 结构域与含有三肽 NPF 的蛋白质的结合已得到很好的描述，但最近的研究表明，EH 结构域也能够与含有 DPF 或 GPF 基序的配体相互作用。我们证明，这三个基序以相似的方式与 EHD1 的 EH 结构域相互作用，由于存在分子间氢键，NPF 基序具有最高的亲和力。DPF 和 GPF 基序的亲和力较弱表明，如果体内形成复合物，它们可能需要高浓度的配体、连续基序的存在和/或特定的侧翼残基。
