[河北省某“献血”村居民丙型肝炎病毒（HCV）分布的基因型调查]
[Investigating genotype of HCV distribution among residents in a "blood donation" village in Hebei Province].
作者信息
Huang Chang-Hong, Zhou Ji-Kun, Liu Li, Jiang Rong-Men, Cao Yan-Qiang, Mu Zhen-Yun, Zhang Yong
机构信息
Hebei Medical University, Shijiazhuang 050017, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Feb;23(1):8-10.
OBJECTIVE
To investigate genotype of HCV infection among residents in a rural village, Hebei Province.
METHODS
Blood samples of the 520 residents were collected, and C/E1 of HCV genome of the 483 samples were obtained by RT-PCR amplifying, and the gene sequences were analyzed and the polygenetic tree were drawn by the software Mega 4.0.
RESULTS
In 483 subjects, HCV-RNA positive objectives are 70, positive prevalence is 14.5%, genotyping for all the samples successfully, including genotypes 1b and 2a, which are 36 (51.4%), 34 (48.6%) respectively.
CONCLUSION
HCV RNA positive rate is 14.5%, which is higher than general people. Subtypes 1b and 2a seemed to be the dominant genotypes of HCV in Zhao county area of Hebei Province.
目的
调查河北省某乡村居民丙型肝炎病毒（HCV）感染的基因型。
方法
采集520名居民的血样，通过逆转录聚合酶链反应（RT-PCR）扩增获得483份样本的HCV基因组C/E1区，分析基因序列并使用Mega 4.0软件绘制系统发育树。
结果
483名受试者中，HCV-RNA阳性者70例，阳性率为14.5%，所有样本均成功进行基因分型，包括1b型和2a型，分别为36例（51.4%）、34例（48.6%）。
结论
HCV RNA阳性率为14.5%，高于普通人群。1b型和2a型似乎是河北省赵县地区HCV的主要基因型。
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