[江苏省宜兴地区丙型肝炎病毒基因分型研究]

[Study on hepatitis C virus genotyping in Yixing area, Jiangsu province].

作者信息

Xu Ke, Deng Xiao-zhao, Ding Wei-liang, Gao Jian, Yu Rong-bin, Diao Zhen-yu, Tan Yong-fei, Zhang Yun

机构信息

Department of Epidemiology, Nanjing Medical University, Nanjing 210029, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2005 Nov;26(11):901-3.

PMID:16676614

Abstract

OBJECTIVE

To investigate the distribution of hepatitis C virus (HCV) genotypes in Yixing, Jiangsu province.

METHODS

Genotypes identification on sera samples were obtained from 158 donors who had already been anti-HCV positive through PCR method with type specific primer designed according to the sequence of 5'non-coding region (5'NCR). 5'NCR was also sequenced and compared with published date. Genotypes distribution was investigated in patients with different sex and clinical types of hepatitis C.

RESULTS

Of the total 158 patients, 95 were HCV RNA positive in which 80 patients having genotype 1b (80/95; 84.4%), 5 patients having genotype 2(5/95; 5.3%), 5 patients with 1b/2 mixed genotypes (5/ 95; 5.3%) and another 5 patients whose genotype undetermined. The difference on the distribution of HCV genotypes was significant between female and male patients (P < 0.05) but not in different kinds of hepatitis C patients.

CONCLUSION

Type 1b was the predominant HCV genotype in Yixing area.

摘要

目的

调查江苏省宜兴市丙型肝炎病毒（HCV）基因型的分布情况。

方法

采用根据5'非编码区（5'NCR）序列设计的型特异性引物，通过PCR方法对158例抗-HCV阳性献血者的血清样本进行基因型鉴定。同时对5'NCR进行测序并与已发表数据进行比较。研究不同性别和临床类型丙型肝炎患者的基因型分布情况。

结果

158例患者中，95例HCV RNA阳性，其中80例为1b基因型（80/95；84.4%），5例为2基因型（5/95；5.3%），5例为1b/2混合基因型（5/95；5.3%），另有5例基因型未确定。HCV基因型分布在女性和男性患者之间存在显著差异（P < 0.05），但在不同类型丙型肝炎患者中无差异。