Zhang Yan, Zhu Zhen, Zhu Shuang-Li, An Hong-Qiu, Yang Zhao-Hui, Guo Xue-Bin, Xu Wen-Bo
National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100050, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Feb;23(1):44-6.
Observe the effect of Ribavirin on reducing the EV71 replication, inactivating EV71 and protecting the RD-A cells against the EV71 infection in vitro.
Using the EV71 isolated from Anhui Fuyang HFMD outbreak, the effect of Ribavirin on RD-A cells during the EV71 infection was observed.
In the experiment of Ribavirin inhibiting the EV71 replication, comparing with the no-Ribavirin-dealed virus control group, the group of 1:640 dilution of Ribavirin delayed the CPE for 2 days, while the normal cell group was growing very well. In the experiment of protecting cell from EV71 infection, comparing with the no-Ribavirin-dealed virus control group, the group of 1:8 dilution of Ribavirin delayed the CPE for 4 days. In the experiment of Ribavirin effect on the inactivation of EV71, the group of 1:40 dilution of Ribavirin delayed the CPE for 2 days comparing with the virus control group.
Ribavirin could inhibit the replication of the EV71 and inactivate the EV71 in vitro. Additionally, Ribavirin could protect RD-A cells from EV 71 infection. The observation will contribute to EV71 infection control and quick medicine therapy.
观察利巴韦林在体外对减少肠道病毒71型（EV71）复制、灭活EV71以及保护RD - A细胞免受EV71感染的作用。
采用从安徽阜阳手足口病疫情中分离出的EV71，观察利巴韦林在EV71感染过程中对RD - A细胞的作用。
在利巴韦林抑制EV71复制的实验中，与未用利巴韦林处理的病毒对照组相比，利巴韦林1:640稀释组使细胞病变效应（CPE）延迟2天，而正常细胞组生长良好。在保护细胞免受EV71感染的实验中，与未用利巴韦林处理的病毒对照组相比，利巴韦林1:8稀释组使CPE延迟4天。在利巴韦林对EV71灭活作用的实验中，与病毒对照组相比，利巴韦林1:40稀释组使CPE延迟2天。
利巴韦林在体外可抑制EV71的复制并灭活EV71。此外，利巴韦林可保护RD - A细胞免受EV71感染。该观察结果将有助于控制EV71感染及快速药物治疗。
