Xu Mei-rong, Chen Yun, Zhou Shao-rong, Chi Ming-ming, Chen Sen-lin, Liu Lei-yu
Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 226001, China.
Zhonghua Zhong Liu Za Zhi. 2009 May;31(5):356-60.
To investigate the mRNA expressions of RASSF1A, Galectin-3 and TPO in papillary thyroid carcinoma and some other thyroid benign lesions, and evaluate their diagnostic significance.
Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of RASSF1A, galectin-3 and TPO in the samples from 73 cases, including 23 cases with papillary thyroid cancer, 16 with nodular goiter, 29 with thyroid adenoma and 5 with Hashimoto's disease.
A statistically significant difference in the mRNA expression of RASSF1A, Galectin-3 and TPO was observed between papillary thyroid carcinoma and follicular benign lesions (P<0.05). However, there was no significant difference among various kinds of benign lesions (P>0.05). A negative correlation of the expression of RASSF1A and Galectin-3 mRNA was found between thyroid benign lesions and malignant ones (P = 0.000). While the mRNA expression of RASSF1A and TPO was positively correlated between benign and malignant lesions (P = 0.028).
Loss of expression of RASSF1A and TPO mRNA but high expression of Galectin-3 mRNA in papillary thyroid carcinoma are common. Therefore, the products of these three genes may be closely related to the development of thyroid papillary carcinoma, and may be used as useful markers in differential diagnosis of papillary thyroid carcinoma from the benign lesions. The results are more reliable if this detection method is used in combination with other techniques.
探讨RASSF1A、半乳糖凝集素-3（Galectin-3）和甲状腺过氧化物酶（TPO）在甲状腺乳头状癌及其他一些甲状腺良性病变中的mRNA表达情况，并评估其诊断意义。
采用逆转录聚合酶链反应（RT-PCR）检测73例样本中RASSF1A、Galectin-3和TPO的mRNA表达，其中包括23例甲状腺乳头状癌、16例结节性甲状腺肿、29例甲状腺腺瘤和5例桥本氏病。
甲状腺乳头状癌与滤泡性良性病变之间RASSF1A、Galectin-3和TPO的mRNA表达存在统计学显著差异（P<0.05）。然而，各类良性病变之间无显著差异（P>0.05）。甲状腺良性病变与恶性病变之间RASSF1A和Galectin-3 mRNA表达呈负相关（P = 0.000）。而RASSF1A和TPO的mRNA表达在良性和恶性病变之间呈正相关（P = 0.028）。
甲状腺乳头状癌中RASSF1A和TPO mRNA表达缺失但Galectin-3 mRNA高表达较为常见。因此，这三个基因的产物可能与甲状腺乳头状癌的发生密切相关，并可作为鉴别甲状腺乳头状癌与良性病变的有用标志物。若将该检测方法与其他技术联合使用，结果将更可靠。
