Nicolas Alain
Institut Curie, Centre de Recherche, UMR7147-CNRS, Université Pierre et Marie Curie, Paris, France.
Methods Mol Biol. 2009;557:27-33. doi: 10.1007/978-1-59745-527-5_3.
Meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the evolutionary conserved Spo11 protein. Along the S. cerevisiae chromosomes, the DSB sites are not evenly distributed and the cleavage frequencies vary 10-100-fold from site to site. Herein are reviewed the methods used in budding yeast to modulate locally and globally the native DSB frequencies, including a powerful method to target Spo11-dependent meiotic DSB in novel chromosomal regions. These methods serve to investigate the control and the mechanism of recombination initiation and modify the natural distribution of meiotic recombination.
减数分裂重组由进化保守的Spo11蛋白形成的DNA双链断裂（DSB）引发。在酿酒酵母染色体上，DSB位点分布不均，不同位点的切割频率相差10至100倍。本文综述了在芽殖酵母中用于局部和全局调节天然DSB频率的方法，包括一种在新的染色体区域靶向Spo11依赖性减数分裂DSB的强大方法。这些方法有助于研究重组起始的调控和机制，并改变减数分裂重组的自然分布。
