Zhang Hai-han, Tang Ming, Chen Hui
College of Life Sciences, Northwest A & F University, Yangling 712100, China.
Huan Jing Ke Xue. 2009 Aug 15;30(8):2432-7.
Determination of the soil microbial community structure in rhizosphere of typical tree species in the Loess Plateau can be of great theoretical significance for correctly assessing the characteristics of soil ecological rehabilitation of the Loess Plateau. In this study,spore density analysis, microbial cultivation and BIOLOG were employed to evaluate the AMF spore density and soil microbial community diversity under four tree species with vesicular-arbuscular mycorrhizae in ecological rehabilitation area of the Loess Plateau, north Shaanxi Province. The results show that the different tree species differed significantly in both soil microbial number and microbial functional diversity, AMF spore density of Hippophae rhamnoides soil is 2.24 times than that of the Robinia pseudoacacia soil,and the rank as following order: Hippophae rhamnoides > Sophora viciifolia > Caragana microphylla > Robinia pseudoacacia . The statistical significant are detected in the bacteria and actinomyces numbers, however, there is no statistical significance in fungi number among the treatments. The principle component analyses indicates that scatter of Caragana microphylla and Hippophae rhamnoides are smaller than that of Sophora viciifolia and Robinia pseudoacacia, these results suggest that the soil community structure strongly varied among the different tree species. Numbers of carbon sources related to the first two components are 14 and 8. Correlation analysis shows that the AMF spore density appeared extremely significantly and positively correlated with the number of bacteria,and the metabolic of amino acids,amines and aromatic compounds, respectively. Moreover,AMF spore density positively correlated with the average well color development (AWCD), nevertheless, no correlations are found among AMF spore density, carboxylic acids,carbohydrates and polymers. These results suggest that AMF spore density is shown to be an important environmental biology parameter used in correctly assessing the soil bacteria metabolic community and diversity under the tree species in ecological rehabilitation of the Loess Plateau, north Shaanxi Province.
确定黄土高原典型树种根际土壤微生物群落结构，对于正确评估黄土高原土壤生态恢复特征具有重要的理论意义。本研究采用孢子密度分析、微生物培养和BIOLOG方法，对陕北黄土高原生态恢复区4种具有泡囊-丛枝菌根的树种下的丛枝菌根真菌（AMF）孢子密度和土壤微生物群落多样性进行了评价。结果表明，不同树种的土壤微生物数量和微生物功能多样性存在显著差异，沙棘土壤的AMF孢子密度是刺槐土壤的2.24倍，排序为：沙棘＞狼牙刺＞小叶锦鸡儿＞刺槐。各处理间细菌和放线菌数量差异有统计学意义，真菌数量差异无统计学意义。主成分分析表明，小叶锦鸡儿和沙棘的离散度小于狼牙刺和刺槐，这些结果表明不同树种间土壤群落结构差异很大。与前两个主成分相关的碳源数量分别为14和8。相关性分析表明，AMF孢子密度与细菌数量、氨基酸、胺类和芳香族化合物的代谢均呈极显著正相关。此外，AMF孢子密度与平均颜色变化率（AWCD）呈正相关，而AMF孢子密度与羧酸、碳水化合物和聚合物之间无相关性。这些结果表明，AMF孢子密度是正确评估陕北黄土高原生态恢复区树种下土壤细菌代谢群落和多样性的一个重要环境生物学参数。
