Shimizu T, Karten H J
Department of Neurosciences, University of California, San Diego, La Jolla 92093-0608.
J Comp Neurol. 1990 Oct 15;300(3):346-69. doi: 10.1002/cne.903000307.
The avian wulst, a laminated "bulge" in the dorsal telencephalon, contains several distinct regions. The posterolateral portion (visual wulst) has been proposed to be an avian equivalent of the mammalian striate cortex. The present study examines specific neurotransmitters and neuropeptides within the visual wulst by immunohistochemical techniques. Antisera and monoclonal antibodies against choline acetyltransferase (ChAT), nicotinic acetylcholine receptor (nAChR), tyrosine hydroxylase (TH), serotonin (5-HT), glutamic acid decarboxylase (GAD), gamma-aminobutyric acid A receptor (GABAAR), cholecystokinin (CCK), substance P (SP), leucine-enkephalin (L-ENK), neurotensin (NT), neuropeptide Y (NPY), somatostatin (SRIF), corticotropin-releasing factor (CRF), and vasoactive intestinal polypeptide (VIP) were used. Somata and neuropil displaying specific immunoreactivity were generally distributed in accordance with the laminar cytoarchitectonic organization of the wulst. The superficial layer of the wulst, the hyperstriatum accessorium, contained the highest densities of TH-, 5-HT-, SP-, NPY-, SRIF-, CRF-, and VIP-positive neuropil in the wulst, whereas the highest density of CCK- and NT-staining was found in the deepest layer of the wulst, the hyperstriatum dorsale. In addition to the traditionally defined four laminae of the wulst, the immunoreactive staining revealed several subregions within each lamina. The most dorsolateral portion of the wulst contained the highest densities of ChAT- and L-ENK-stained fibers in the wulst, as well as moderately dense staining of neuropil for 5-HT-, TH-, SP-, and CCK-like immunoreactivity. The nAChR-immunoreactivity was faint and distributed rather uniformly throughout the wulst. The results suggest that the wulst consists of multiple regional variations within layers comparable to laminar variations found within different cytoarchitectonic areas of the mammalian neocortex.
鸟类的上纹体是背侧端脑的一个分层“隆起”，包含几个不同的区域。后外侧部分（视觉上纹体）被认为相当于哺乳动物的纹状皮质。本研究通过免疫组织化学技术检测视觉上纹体内的特定神经递质和神经肽。使用了针对胆碱乙酰转移酶（ChAT）、烟碱型乙酰胆碱受体（nAChR）、酪氨酸羟化酶（TH）、5-羟色胺（5-HT）、谷氨酸脱羧酶（GAD）、γ-氨基丁酸A受体（GABAAR）、胆囊收缩素（CCK）、P物质（SP）、亮氨酸脑啡肽（L-ENK）、神经降压素（NT）、神经肽Y（NPY）、生长抑素（SRIF）、促肾上腺皮质激素释放因子（CRF）和血管活性肠肽（VIP）的抗血清和单克隆抗体。显示特定免疫反应性的胞体和神经纤维网通常根据上纹体的分层细胞构筑组织分布。上纹体的表层，即副新纹状体，在上纹体中含有最高密度的TH、5-HT、SP、NPY、SRIF、CRF和VIP阳性神经纤维网，而CCK和NT染色的最高密度则出现在上纹体的最深层，即背侧新纹状体。除了传统定义的上纹体的四层外，免疫反应性染色还揭示了每层内的几个亚区域。上纹体最背外侧部分在上纹体中含有最高密度的ChAT和L-ENK染色纤维，以及5-HT、TH、SP和CCK样免疫反应性的中等密度神经纤维网染色。nAChR免疫反应性较弱，在上纹体中分布较为均匀。结果表明，上纹体由各层内的多个区域差异组成，类似于在哺乳动物新皮质不同细胞构筑区域内发现的分层差异。
