University Medical Center Maastricht, The Netherlands.
Am J Obstet Gynecol. 2010 Jan;202(1):77.e1-9. doi: 10.1016/j.ajog.2009.07.058. Epub 2009 Oct 4.
We hypothesized that fetal lipopolysaccharide exposures to the chorioamnion, lung, or gut would induce distinct systemic inflammatory responses.
Groups of 5-7 time-mated ewes were used to surgically isolate the fetal respiratory and the gastrointestinal systems from the amniotic compartment. Outcomes were assessed at 124 days gestational age, 2 days and 7 days after lipopolysaccharide (10 mg, Escherichia coli 055:B5) or saline solution infusions into the fetal airways or amniotic fluid.
Lipopolysaccharide induced systemic inflammatory changes in all groups in the blood, lung, liver, and thymic lymphocytes. Changes in lymphocytes in the posterior mediastinal lymph node draining lung and gut, occurred only after direct contact of lipopolysaccharide with the fetal lung or gut.
Fetal systemic inflammatory responses occurred after chorioamnion, lung, or gut exposures to lipopolysaccharide. The organ responses differed based on route of the fetal exposure.
我们假设胎儿接触绒毛膜羊膜炎、肺或肠道中的脂多糖会引起不同的全身炎症反应。
将 5-7 只同期发情的母羊分为几组，通过手术将胎儿呼吸系统和胃肠道与羊膜腔隔离开来。在妊娠 124 天时，在胎儿气道或羊水中注入脂多糖（10mg，大肠杆菌 055:B5）或生理盐水后 2 天和 7 天，评估结局。
脂多糖诱导了所有组的血液、肺、肝和胸腺淋巴细胞中的全身炎症变化。仅在脂多糖直接接触胎儿肺或肠道后，后纵隔淋巴结引流肺和肠道的淋巴细胞才会发生变化。
胎儿在接触绒毛膜羊膜炎、肺或肠道中的脂多糖后会发生全身炎症反应。胎儿暴露途径的不同会导致器官反应的差异。
