Kim N P, Akhmerov R N, Makhmudov E S
Ukr Biokhim Zh (1978). 1990 Sep-Oct;62(5):67-72.
In unwashed mitochondria the oxidation of L-lactate (with NAD+) proceeds in presence of the added lactate dehydrogenase. The respiration is characterized by the high rate in state 4 and is stimulated by ADP. This process takes place in unwashed mitochondria and homogenate of the heart in absence of added lactate dehydrogenase. Oxidation of lactate with NAD+ is inhibited by rotenone. It has been also revealed that the oxidation of glutamate is insufficiently altered in presence of lactate (with NAD+) in unwashed mitochondria as compared with the washed ones. It is supposed that the stimulating effect of lactate with NAD+ on the mitochondria respiration is not so much a result of the membrane-damaged action as a result of oxidation of lactate dehydrogenase reaction products: phosphorylative oxidation of pyruvate and nonconjugated oxidation of NADH. Utilization of these products takes place in the main respiratory chain, including its first stage.
在未洗涤的线粒体中，L-乳酸（与NAD+）的氧化在添加乳酸脱氢酶的情况下进行。呼吸作用的特点是在状态4时速率较高，并受到ADP的刺激。这个过程在未洗涤的线粒体和心脏匀浆中，在没有添加乳酸脱氢酶的情况下发生。用NAD+氧化乳酸受到鱼藤酮的抑制。还发现，与洗涤过的线粒体相比，在未洗涤的线粒体中，乳酸（与NAD+）存在时谷氨酸的氧化变化不大。据推测，NAD+乳酸对线粒体呼吸的刺激作用与其说是膜损伤作用的结果，不如说是乳酸脱氢酶反应产物氧化的结果：丙酮酸的磷酸化氧化和NADH的非共轭氧化。这些产物的利用发生在主要呼吸链中，包括其第一阶段。
