Adeyemi E O, Chadwick V S, Hodgson H J
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
J Pharm Pharmacol. 1990 Jul;42(7):487-90. doi: 10.1111/j.2042-7158.1990.tb06601.x.
In view of the potential role of released polymorphonuclear leucocyte elastase in causing tissue damage, the effect of commonly used anti-inflammatory drugs on elastase release from neutrophils has been studied in-vitro. Elastase release from neutrophils exposed to the synthetic bacterial cell wall peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine (10(-6) M) was quantitated using a radiometric immunoassay and a functional assay of elastase. Prednisolone and non-steroidal anti-inflammatory drugs inhibited elastase release at concentrations from 0.1 mM-0.1 nM. No inhibition by sulphosalicylic acid, D-penicillamine or chloroquine sulphate was observed. The clinical relevance of these findings is discussed.
鉴于释放的多形核白细胞弹性蛋白酶在造成组织损伤方面的潜在作用，已在体外研究了常用抗炎药对中性粒细胞弹性蛋白酶释放的影响。使用放射免疫测定法和弹性蛋白酶功能测定法对暴露于合成细菌细胞壁肽N-甲酰-L-蛋氨酰-L-亮氨酰-L-苯丙氨酸（10^(-6) M）的中性粒细胞的弹性蛋白酶释放进行了定量。泼尼松龙和非甾体抗炎药在0.1 mM至0.1 nM的浓度下抑制弹性蛋白酶释放。未观察到磺基水杨酸、D-青霉胺或硫酸氯喹的抑制作用。讨论了这些发现的临床相关性。
