Center for Clinical and Experimental Allergology, IDI-IRCCS, Rome, Italy.
Ital J Pediatr. 2009 Oct 5;35(1):29. doi: 10.1186/1824-7288-35-29.
Identification, characterization, and purification of allergens are essential for the structural and immunologic studies needed to understand how these molecules induce specific IgE antibody production by the human immune system. Advances in molecular biology techniques have led to the production of recombinant allergens having constant properties, allowing detection of specific IgE directed against different molecular components of an allergenic source. Presence of homologous allergens in different sources is the reason for cross-reaction. Molecule-based diagnostic tools can lead to better interpretation of poly-sensitizations, observed by ST and in vitro tests using allergenic extracts as they were made before. Some examples IgE sensitization to major genuine allergens and panallergens will be presented.
鉴定、描述和提纯变应原对于结构和免疫研究至关重要，这有助于理解这些分子如何诱导人类免疫系统产生特异性 IgE 抗体。分子生物学技术的进步导致了具有恒定特性的重组变应原的产生，从而可以检测针对变应原来源不同分子成分的特异性 IgE。不同来源的同源变应原是交叉反应的原因。基于分子的诊断工具可以更好地解释多敏化现象，这是在使用变应原提取物进行 ST 和体外测试时观察到的，而这些提取物是在之前制作的。将介绍一些 IgE 对主要天然变应原和泛变应原致敏的例子。
