Department of Chemical Engineering, Graduate School of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan.
J Biosci Bioeng. 2009 Nov;108(5):394-9. doi: 10.1016/j.jbiosc.2009.05.007.
Analyses of the nitrite reductase gene diversities (nirK and nirS) in an activated sludge community fed with both nitrite and glucose were conducted. Results suggest that the topology of nirK and nirS gene fragment-based phylogenetic trees is influenced more by the available electron acceptor than by the carbon source. A denitrification reactor was operated for 53 days and a clone library constructed when the denitrifying communities in the SBR were supplied with both nitrite and glucose. Half of the nirK and nearly all the nirS gene fragments formed a cluster that was separate from a cluster containing nirK and nirS sequences derived from other communities in nitrate-fed reactors. On the other hand, nirK and nirS fragments obtained with glucose as the carbon source were similar to those detected in communities fed with other carbon sources.
分析了同时含有亚硝酸盐和葡萄糖的活性污泥群落中的亚硝酸盐还原酶基因多样性（nirK 和 nirS）。结果表明，nirK 和 nirS 基因片段基于系统发育树的拓扑结构更多地受到可用电子受体的影响，而不是碳源。在 SBR 中同时提供亚硝酸盐和葡萄糖的情况下，反硝化反应器运行了 53 天，并构建了一个克隆文库。nirK 和 nirS 基因片段的一半形成了一个与包含来自硝酸盐喂养反应器中其他群落的 nirK 和 nirS 序列的聚类分开的聚类。另一方面，以葡萄糖作为碳源获得的 nirK 和 nirS 片段与检测到的以其他碳源为食的群落中的片段相似。
