Kahri A I
J Cell Biol. 1968 Jan;36(1):181-95.
The ultrastructure of the mitochondria of the cultured cortical cells of rat adrenals was studied. In vivo it was found that the zona fasciculata mitochondria have vesicular internal structure. 600-A vesicles appear free in the matrix or as protrusions of the inner mitochondrial membrane. In tissue cultures of the fetal and newborn rat adrenal cortex it was seen that ACTH induces transformation of the tubulo-vesicular internal structure of the mitochondria to 600-A vesicles. Actinomycin D and puromycin inhibited this transformation if they were added with ACTH. When added alone, these inhibitors of protein synthesis induced no change in the ultrastructure of the mitochondria in cultured cortical cells of rat adrenals.
对大鼠肾上腺皮质培养细胞的线粒体超微结构进行了研究。在体内发现,束状带线粒体具有泡状内部结构。600埃的小泡在基质中游离出现,或作为线粒体内膜的突起。在胎鼠和新生鼠肾上腺皮质的组织培养中,观察到促肾上腺皮质激素(ACTH)可诱导线粒体的管状-泡状内部结构转变为600埃的小泡。如果放线菌素D和嘌呤霉素与ACTH一起添加,则会抑制这种转变。单独添加时,这些蛋白质合成抑制剂不会引起大鼠肾上腺皮质培养细胞中线粒体超微结构的变化。