Zhou Dan-Lei, Yan Dan, Li Bao-Cai, Wu Yan-Shu, Xiao Xiao-He
China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, China.
Yao Xue Xue Bao. 2009 Jun;44(6):640-4.
This study is to investigate the effect of Cordyceps sinensis and its cultured mycelia on growth and metabolism of Escherichia coli, and microcalorimetric method was carried out to evaluate its biological activity. The study will provide the basis for the quality control of Cordyceps sinensis. Experimental result will show the effect of natural Cordyceps sinensis and its cultured mycelia on growth and metabolism of Escherichia coli, with index of P(1max) and effective rate (E) by microcalorimetry, the data of experiment were studied by cluster analysis. The results showed that Cordyceps sinensis and its cultured mycelia not only can promote growth and metabolism of Escherichia coli but also can regulate the balance of intestinal microecology efficiently. When the concentrations of samples > 6.0 mg mL(-1), natural Cordyceps sinensis can promote the growth and metabolism of Escherichia coli efficiently (P < 0.05) compared with the control group, and have better dose-effect relationship with concentration (r > 0.9), its cultured mycelia does not show conspicuous auxoaction (P > 0.05) and have not dose-effect relationship with concentration (r < 0.6); when the concentration of samples < 6.0 mg mL(-1), all samples does not show conspicuous auxoaction (P > 0.05). Natural Cordyceps sinensis and its cultured mycelia can be distinguished by cluster analysis. Microcalorimetry has a good prospect on the quality evaluation of the traditional Chinese medicine.
本研究旨在探讨冬虫夏草及其人工培养菌丝体对大肠杆菌生长代谢的影响，并采用微量量热法评价其生物活性。该研究将为冬虫夏草的质量控制提供依据。实验结果将显示天然冬虫夏草及其人工培养菌丝体对大肠杆菌生长代谢的影响，通过微量量热法得出P(1max)指数和有效率(E)，并对实验数据进行聚类分析。结果表明，冬虫夏草及其人工培养菌丝体不仅能促进大肠杆菌的生长代谢，还能有效调节肠道微生态平衡。当样品浓度>6.0 mg mL(-1)时，天然冬虫夏草与对照组相比能有效促进大肠杆菌的生长代谢(P<0.05)，且与浓度具有较好的剂量效应关系(r>0.9)，其人工培养菌丝体未表现出明显的促进作用(P>0.05)，与浓度无剂量效应关系(r<0.6)；当样品浓度<6.0 mg mL(-1)时，所有样品均未表现出明显的促进作用(P>0.05)。通过聚类分析可区分天然冬虫夏草及其人工培养菌丝体。微量量热法在中药质量评价方面具有良好的应用前景。
