Roberts R C, DiFiglia M
Laboratory of Cellular Neurobiology, Massachusetts General Hospital-East, Charlestown 02129.
Brain Res. 1990 Nov 5;532(1-2):151-9. doi: 10.1016/0006-8993(90)91755-6.
Neurons in long-term striatal grafts were examined to determine if they retain the neurotransmitter characteristics of cells in younger grafts. In addition, calbindin-d28k, a ubiquitous marker of medium spiny neurons, was used to examine the overall frequency and ultrastructural characteristics of spiny neurons in the older grafts. Grafts from 17-day fetal striata were injected into the quinolinic acid-lesioned caudate nucleus in 5 adult rats. After 16 months, the neostriatum was processed for the localization of immunoreactive GABA, calbindin, enkephalin and NADPH-diaphorase (-d) activity. The proportions of GABA-, enkephalin- and NADPH-d-labeled neurons to total Nissl-stained neurons in the 16-month-old grafts (25 +/- 6, 13 +/- 4, and 3 +/- 3, respectively) were similar to findings in 2-month-old grafts. Calbindin-positive cells formed the highest proportion (36.3 +/- 3) of labeled neurons in the older grafts. Nuclear and spine morphology of immunoreactive calbindin cells varied more in the grafts than in host caudate. Results show that there is long-term survival and stability of GABA, enkephalin and NADPH-d cell populations in the grafts and that some grafted spiny neurons may exhibit altered phenotype from those of host striatum.
对长期纹状体移植中的神经元进行了检查，以确定它们是否保留了较年轻移植细胞的神经递质特征。此外，钙结合蛋白-d28k是一种中等棘状神经元的普遍标记物，用于检查较老移植中棘状神经元的总体频率和超微结构特征。将来自17天龄胎儿纹状体的移植物注射到5只成年大鼠经喹啉酸损伤的尾状核中。16个月后，对新纹状体进行处理，以定位免疫反应性γ-氨基丁酸（GABA）、钙结合蛋白、脑啡肽和还原型辅酶II黄递酶（NADPH-d）活性。在16个月大的移植物中，GABA、脑啡肽和NADPH-d标记神经元占总尼氏染色神经元的比例（分别为25±6、13±4和3±3）与2个月大移植物中的结果相似。在较老的移植物中，钙结合蛋白阳性细胞占标记神经元的比例最高（36.3±3）。与宿主尾状核相比，移植物中免疫反应性钙结合蛋白细胞的核和棘形态变化更大。结果表明，移植物中GABA、脑啡肽和NADPH-d细胞群体具有长期存活和稳定性，并且一些移植的棘状神经元可能表现出与宿主纹状体不同的表型。
